Human BPGM Recombinant Protein (RPPB1451)
- SKU:
- RPPB1451
- Product Type:
- Recombinant Protein
- Species:
- Human
- Uniprot:
- P07738
- Research Area:
- Enzymes
Description
| Product Name: | Human BPGM Recombinant Protein |
| Product Code: | RPPB1451 |
| Size: | 20µg |
| Species: | Human |
| Target: | BPGM |
| Synonyms: | Bisphosphoglycerate mutase, EC 5.4.2.4, BPGM, 2,3-bisphosphoglycerate mutase erythrocyte, 2,3-bisphosphoglycerate synthase, BPG-dependent PGAM. |
| Source: | Escherichia Coli |
| Physical Appearance: | Sterile Filtered clear colorless solution. |
| Formulation: | The BPGM solution (0.5mg/ml) contains 20mM Tris-HCl (pH 8.0), 1mM DTT and 10% glycerol. |
| Stability: | Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles. |
| Purity: | Greater than 95% as determined by SDS-PAGE. |
| Amino Acid Sequence: | MSKYKLIMLR HGEGAWNKEN RFCSWVDQKL NSEGMEEARN CGKQLKALNF EFDLVFTSVL NRSIHTAWLI LEELGQEWVP VESSWRLNERHYGALIGLNR EQMALNHGEE QVRLWRRSYN VTPPPIEESH PYYQEIYNDR RYKVCDVPLD QLPRSESLKD VLERLLPYWN ERIAPEVLRG KTILISAHGN SSRALLKHLE GISDEDIINI TLPTGVPILL ELDENLRAVG PHQFLGDQEA IQAAIKKVED QGKVKQAKKL EHHHHHH |
BPGM is found at high concentrations in red blood cells where it binds to and decreases the oxygen affinity of hemoglobin. PGM deficiency increases the oxygen affinity of cells. BPGM is a multifunctional enzyme that catalyzes 2,3-DPG synthesis through its synthetase activity, and 2,3-DPG degradation using its phosphatase activity. BPGM has phosphoglycerate phosphomutase activity. Mutations in BPGM cause hemolytic anemia. BPGM catalyzes the reaction of EC 5.4.2.1 (mutase) and EC 3.1.3.13 (phosphatase), but with a reduced activity.
BPGM Human Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 267 amino acids (1-259 a.a.) and having a molecular mass of 31 kDa. The BPGM is fused to an 8 amino acid His Tag at C-Terminus and purified by proprietary chromatographic techniques.
| UniProt Protein Function: | BPGM: Plays a major role in regulating hemoglobin oxygen affinity by controlling the levels of its allosteric effector 2,3- bisphosphoglycerate (2,3-BPG). Also exhibits mutase (EC 5.4.2.1) and phosphatase (EC 3.1.3.13) activities. Defects in BPGM are the cause of bisphosphoglycerate mutase deficiency (BPGMD). A disease characterized by hemolytic anemia, splenomegaly, cholelithiasis and cholecystitis. Belongs to the phosphoglycerate mutase family. BPG- dependent PGAM subfamily. |
| UniProt Protein Details: | Protein type:EC 5.4.2.4; EC 3.1.3.13; Carbohydrate Metabolism - glycolysis and gluconeogenesis; Phosphatase (non-protein); EC 5.4.2.11; Isomerase Chromosomal Location of Human Ortholog: 7q33 Molecular Function:phosphoglycerate mutase activity; bisphosphoglycerate mutase activity; bisphosphoglycerate phosphatase activity Biological Process: erythrocyte development; glycolysis; dephosphorylation; carbohydrate metabolic process; respiratory gaseous exchange Disease: Bisphosphoglycerate Mutase Deficiency |
| NCBI Summary: | 2,3-diphosphoglycerate (2,3-DPG) is a small molecule found at high concentrations in red blood cells where it binds to and decreases the oxygen affinity of hemoglobin. This gene encodes a multifunctional enzyme that catalyzes 2,3-DPG synthesis via its synthetase activity, and 2,3-DPG degradation via its phosphatase activity. The enzyme also has phosphoglycerate phosphomutase activity. Deficiency of this enzyme increases the affinity of cells for oxygen. Mutations in this gene result in hemolytic anemia. Multiple alternatively spliced variants, encoding the same protein, have been identified. [provided by RefSeq, Sep 2009] |
| UniProt Code: | P07738 |
| NCBI GenInfo Identifier: | 130350 |
| NCBI Gene ID: | 669 |
| NCBI Accession: | P07738.2 |
| UniProt Related Accession: | P07738 |
| Molecular Weight: | 30kDa |
| NCBI Full Name: | Bisphosphoglycerate mutase |
| NCBI Synonym Full Names: | bisphosphoglycerate mutase |
| NCBI Official Symbol: | BPGM�� |
| NCBI Official Synonym Symbols: | DPGM; ECYT8�� |
| NCBI Protein Information: | bisphosphoglycerate mutase |
| UniProt Protein Name: | Bisphosphoglycerate mutase |
| UniProt Synonym Protein Names: | 2,3-bisphosphoglycerate mutase, erythrocyte; 2,3-bisphosphoglycerate synthase (EC:3.1.3.13, EC:5.4.2.11); 2,3-diphosphoglycerate mutase; DPGM; BPG-dependent PGAM |
| Protein Family: | Bisphosphoglycerate mutase |
| UniProt Gene Name: | BPGM�� |
| UniProt Entry Name: | PMGE_HUMAN |
