Human CBR1 Recombinant Protein (RPPB1475)
- SKU:
- RPPB1475
- Product Type:
- Recombinant Protein
- Species:
- Human
- Uniprot:
- P16152
- Research Area:
- Enzymes
Description
| Product Name: | Human CBR1 Recombinant Protein |
| Product Code: | RPPB1475 |
| Size: | 20µg |
| Species: | Human |
| Target: | CBR1 |
| Synonyms: | CBR, hCBR1, SDR21C1, CBR1, Carbonyl reductase [NADPH] 1, NADPH-dependent carbonyl reductase 1, Prostaglandin-E(2) 9-reductase, Prostaglandin 9-ketoreductase, 15-hydroxyprostaglandin dehydrogenase [NADP+], CRN. |
| Source: | Escherichia Coli |
| Physical Appearance: | Sterile filtered colorless solution. |
| Formulation: | The CBR1 protein contains 20mM Tris-HCl buffer pH-8.5, and 10% glycerol. |
| Stability: | Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid multiple freeze-thaw cycles. |
| Purity: | Greater than 95.0% as determined by analysis by SDS-PAGE. |
| Amino Acid Sequence: | MSSGIHVALV TGGNKGIGLA IVRDLCRLFS GDVVLTARDV TRGQAAVQQL QAEGLSPRFH QLDIDDLQSI RALRDFLRKE YGGLDVLVNN AGIAFKVADP TPFHIQAEVT MKTNFFGTRD VCTELLPLIK PQGRVVNVSS IMSVRALKSC SPELQQKFRS ETITEEELVG LMNKFVEDTK KGVHQKEGWP SSAYGVTKIG VTVLSRIHAR KLSEQRKGDK ILLNACCPGW VRTDMAGPKA TKSPEEGAET PVYLALLPPD AEGPHGQFVS EKRVEQW |
CBR1 is one of numerous monomeric, NADPH-dependent oxidoreductases having ubiquistly specificity for carbonyl compounds. CBR1 is broadly distributed in human tissues. CBR1 metabolizes toxic environmental quinones and pharmacological relevant substrates. CBR1 converts prostaglandin E2 to prostaglandin F2-alpha.
Recombinant Human CBR1 produced in E.Coli is a single, non-glycosylated polypeptide chain containing 277 amino acids (1-277 a.a) and having a molecular mass of 30 kDa. CBR1 is purified by proprietary chromatographic techniques.
| UniProt Protein Function: | CBR1: NADPH-dependent reductase with broad substrate specificity. Catalyzes the reduction of a wide variety of carbonyl compounds including quinones, prostaglandins, menadione, plus various xenobiotics. Catalyzes the reduction of the antitumor anthracyclines doxorubicin and daunorubicin to the cardiotoxic compounds doxorubicinol and daunorubicinol. Can convert prostaglandin E2 to prostaglandin F2-alpha. Can bind glutathione, which explains its higher affinity for glutathione-conjugated substrates. Catalyzes the reduction of S-nitrosoglutathione. Belongs to the short-chain dehydrogenases/reductases (SDR) family. |
| UniProt Protein Details: | Protein type:EC 1.1.1.189; Lipid Metabolism - arachidonic acid; EC 1.1.1.197; Oxidoreductase; EC 1.1.1.184 Chromosomal Location of Human Ortholog: 21q22.13 Cellular Component: cytosol; vesicle Molecular Function:oxidoreductase activity, acting on NADH or NADPH, quinone or similar compound as acceptor; prostaglandin-E2 9-reductase activity; 15-hydroxyprostaglandin dehydrogenase (NADP+) activity; carbonyl reductase (NADPH) activity Biological Process: epithelial cell differentiation; vitamin K metabolic process; cyclooxygenase pathway; arachidonic acid metabolic process; drug metabolic process |
| NCBI Summary: | The protein encoded by this gene belongs to the short-chain dehydrogenases/reductases (SDR) family, which function as NADPH-dependent oxidoreductases having wide specificity for carbonyl compounds, such as quinones, prostaglandins, and various xenobiotics. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Nov 2013] |
| UniProt Code: | P16152 |
| NCBI GenInfo Identifier: | 118519 |
| NCBI Gene ID: | 873 |
| NCBI Accession: | P16152.3 |
| UniProt Secondary Accession: | P16152,Q3LHW8, B2RBZ7, B4DFK7, |
| UniProt Related Accession: | P16152 |
| Molecular Weight: | 277 |
| NCBI Full Name: | Carbonyl reductase |
| NCBI Synonym Full Names: | carbonyl reductase 1 |
| NCBI Official Symbol: | CBR1�� |
| NCBI Official Synonym Symbols: | CBR; hCBR1; SDR21C1�� |
| NCBI Protein Information: | carbonyl reductase [NADPH] 1; carbonyl reductase [NADPH] 1; carbonyl reductase (NADPH) 1; prostaglandin 9-ketoreductase; prostaglandin-E(2) 9-reductase; NADPH-dependent carbonyl reductase 1; 15-hydroxyprostaglandin dehydrogenase; short chain dehydrogenase/reductase family 21C, member 1 |
| UniProt Protein Name: | Carbonyl reductase [NADPH] 1 |
| UniProt Synonym Protein Names: | 15-hydroxyprostaglandin dehydrogenase [NADP(+)] (EC:1.1.1.197); NADPH-dependent carbonyl reductase 1; Prostaglandin 9-ketoreductase; Prostaglandin-E(2) 9-reductase (EC:1.1.1.189) |
| Protein Family: | Carbonyl reductase |
| UniProt Gene Name: | CBR1�� |
| UniProt Entry Name: | CBR1_HUMAN |
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