Description
| Product Name: | Human CENPH Recombinant Protein |
| Product Code: | RPPB3146 |
| Size: | 20µg |
| Species: | Human |
| Target: | CENPH |
| Synonyms: | Centromere protein H, Interphase centromere complex protein 35, CENP-H, NNF1, PMF1, ICEN35, Kinetochore protein CENP-H. |
| Source: | Escherichia Coli |
| Physical Appearance: | Sterile Filtered colorless solution. |
| Formulation: | The CENPH solution (1mg/ml) contains 20mM Tris-HCl buffer (pH 8.0) and 10% glycerol. |
| Stability: | Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles. |
| Purity: | Greater than 90% as determined by SDS-PAGE. |
| Amino Acid Sequence: | MGSSHHHHHH SSGLVPRGSH MLNKLIMKSQ QESWDLEEKL LDIRKKRLQL KQASESKLLE IQTEKNKQKI DLDSMENSER IKIIRQNLQM EIKITTVIQH VFQNLILGSK VNWAEDPALK EIVLQLEKNV DMM |
CENPH is a member of the centromere protein H family. CENPH protein is a component of the CENPA-NAC (nucleosome-associated) complex which has a vital part in assembly of kinetochore proteins. The CENPA-NAC complex utilizes the CENPA-CAD (nucleosome distal) complex and is involved in integration of freshly synthesized CENPA into centromeres.
CENPH Human Recombinant produced in E.coli is a single, non-glycosylated polypeptide chain containing 133 amino acids (136-247) and having a molecular mass of 15.5 kDa.The CENPH is fused to a 20 amino acid His-Tag at N-terminus and purified by proprietary chromatographic techniques.
| UniProt Protein Function: | CENPH: Component of the CENPA-NAC (nucleosome-associated) complex, a complex that plays a central role in assembly of kinetochore proteins, mitotic progression and chromosome segregation. The CENPA-NAC complex recruits the CENPA-CAD (nucleosome distal) complex and may be involved in incorporation of newly synthesized CENPA into centromeres. Required for chromosome congression and efficiently align the chromosomes on a metaphase plate. Belongs to the centromere protein H family. |
| UniProt Protein Details: | Protein type:Cell cycle regulation Chromosomal Location of Human Ortholog: 5p15.2 Cellular Component: cytosol; kinetochore; nucleoplasm; nucleus Molecular Function:kinetochore binding; protein binding Biological Process: DNA replication-independent nucleosome assembly at centromere; kinetochore organization and biogenesis; sister chromatid cohesion |
| NCBI Summary: | Centromere and kinetochore proteins play a critical role in centromere structure, kinetochore formation, and sister chromatid separation. The protein encoded by this gene colocalizes with inner kinetochore plate proteins CENP-A and CENP-C in both interphase and metaphase. It localizes outside of centromeric heterochromatin, where CENP-B is localized, and inside the kinetochore corona, where CENP-E is localized during prometaphase. It is thought that this protein can bind to itself, as well as to CENP-A, CENP-B or CENP-C. Multimers of the protein localize constitutively to the inner kinetochore plate and play an important role in the organization and function of the active centromere-kinetochore complex. [provided by RefSeq, Jul 2008] |
| UniProt Code: | Q9H3R5 |
| NCBI GenInfo Identifier: | 74733576 |
| NCBI Gene ID: | 64946 |
| NCBI Accession: | Q9H3R5.1 |
| UniProt Secondary Accession: | Q9H3R5,A8K3Y1, |
| UniProt Related Accession: | Q9H3R5 |
| Molecular Weight: | 28,481 Da |
| NCBI Full Name: | Centromere protein H |
| NCBI Synonym Full Names: | centromere protein H |
| NCBI Official Symbol: | CENPH�� |
| NCBI Protein Information: | centromere protein H |
| UniProt Protein Name: | Centromere protein H |
| UniProt Synonym Protein Names: | Interphase centromere complex protein 35 |
| UniProt Gene Name: | CENPH�� |
| UniProt Entry Name: | CENPH_HUMAN |
