The Human CFH (Complement Factor H) ELISA Kit is a specialized assay designed for the precise quantitative detection of CFH levels in various biological samples. Complement Factor H is a key regulator of the alternative complement pathway, crucial for immune homeostasis, host defense, and preventing excessive complement activation. CFH serves as a vital factor in protecting host cells from complement-mediated damage, modulation of complement activity, and regulation of inflammation. Understanding CFH levels is pivotal for unraveling immune responses, complement system function, and pathogenic mechanisms related to complement dysregulation. Accurate measurement of CFH is essential for investigating its regulatory roles in immune responses, inflammatory processes, and complement-mediated diseases. With outstanding sensitivity and specificity, Assay Genie's CFH ELISA Kit ensures reliable and reproducible results. Manufactured under stringent quality controls, this kit offers robust performance and user-friendly protocols, making it an excellent choice for both basic research and clinical studies. Researchers can trust Assay Genie's CFH ELISA Kit for precise quantification of this critical complement factor in their investigations.
Product Name:
Human CFH (Complement Factor H) ELISA Kit
SKU:
AEES00093
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Sandwich
Assay type:
Sandwich-ELISA
Assay time:
3 h 30 min
Sensitivity:
1.88 ng/mL
Detection range:
3.13-200 ng/mL
Reovery:
80%-120%
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to the target protein. Standards or samples are added to the micro ELISA plate wells and bind to the immobilized antibody. A biotinylated detection antibody specific to the target protein is then added, followed by Avidin-Horseradish Peroxidase (HRP) conjugate. Free components are washed away. The substrate solution is added to each well, resulting in a color change. Only wells containing the target protein, detection antibody, and HRP conjugate will develop a blue color. The reaction is terminated by the addition of stop solution, resulting in a yellow color. The optical density (OD) is measured at 450 nm ± 2 nm. The OD value is directly proportional to the concentration of the target protein in the sample and is determined using a standard curve.
