The Human CHGB (Chromogranin B) ELISA Kit is specially designed for the quantitative detection of chromogranin B levels in human serum, plasma, and cell culture supernatants. This kit offers superior sensitivity and specificity, ensuring accurate and dependable results for various research applications.Chromogranin B is a key protein involved in neuroendocrine function, playing a crucial role in hormone storage and secretion. Abnormal levels of chromogranin B have been associated with various neuroendocrine tumors and other diseases, making it a valuable biomarker for studying these conditions and developing targeted therapies.
With its high-quality components and user-friendly protocols, the Human CHGB ELISA Kit is a valuable tool for researchers studying neuroendocrine disorders and related conditions. Trust Assay Genie for reliable and reproducible results in your research endeavors.
Product Name:
Human CHGB (Chromogranin B) ELISA Kit
SKU:
HUES01967
Target:
Human CHGB (Chromogranin B)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
37.50 pg/mL
Detection range:
62.50-4000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human CHGB. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human CHGB and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human CHGB, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human CHGB. You can calculate the concentration of Human CHGB in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
87-100
89-101
95-109
Average (%)
94
95
101
1:4
Range (%)
89-105
82-92
85-98
Average (%)
96
87
90
1:8
Range (%)
88-104
81-93
83-93
Average (%)
96
87
88
1:16
Range (%)
93-106
85-100
87-100
Average (%)
99
92
95
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
89-101
96
EDTA plasma (n=5)
85-98
91
Cell culture media (n=5)
84-97
90
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
186.91
350.82
1795.25
180.09
319.24
1941.2
Standard deviation
12.6
17.12
80.07
11.38
15.96
89.3
C V (%)
6.74
4.88
4.46
6.32
5.0
4.6
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human CHGB concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human CHGB in samples. No significant cross-reactivity or interference between Human CHGB and analogues was observed.
