The Human Col15 (Collagen Type XV) CLIA Kit is a highly sensitive and specific assay used for the accurate detection of Col15 levels in human samples including serum, plasma, and cell culture supernatants. This kit provides reliable and reproducible results, making it an ideal solution for various research applications.Collagen Type XV is a critical component of the extracellular matrix, playing a vital role in tissue structure and function. Dysregulation of Col15 has been linked to various pathological conditions such as connective tissue disorders, muscular dystrophy, and certain cancers.
Therefore, measuring Col15 levels can provide valuable insights into disease mechanisms and potential therapeutic targets.With its advanced technology and user-friendly design, the Human Col15 CLIA Kit from Assaygenie is a valuable tool for researchers studying extracellular matrix dynamics and exploring novel therapeutic interventions. Trust in the accuracy and precision of this kit to propel your research forward in the field of collagen biology and related disorders.
Product Name:
Human COL15 (Collagen Type XV) CLIA Kit
SKU:
HUES00482
Target:
Human COL15 (Collagen Type XV)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
37.50 pg/mL
Detection range:
62.50-4000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human COL15. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human COL15 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human COL15, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human COL15. You can calculate the concentration of Human COL15 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
91-104
91-103
86-99
Average (%)
97
96
93
1:4
Range (%)
102-116
88-102
95-108
Average (%)
108
94
102
1:8
Range (%)
95-110
85-99
96-110
Average (%)
100
91
104
1:16
Range (%)
97-112
92-104
90-104
Average (%)
105
98
96
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
93-106
99
EDTA plasma (n=5)
92-110
100
Cell culture media (n=5)
99-113
106
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
210.1
356.42
1457.03
204.53
371.55
1344.92
Standard deviation
25.53
40.7
107.53
24.2
39.79
117.01
C V (%)
12.15
11.42
7.38
11.83
10.71
8.7
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human COL15 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human COL15 in samples. No significant cross-reactivity or interference between Human COL15 and analogues was observed.
