The Human COL6 (Collagen Type VI) ELISA Kit is specifically engineered for the precise measurement of collagen type VI levels in human serum, plasma, and cell culture supernatants. With its exceptional sensitivity and specificity, this kit delivers dependable and consistent results, making it perfect for various research endeavors.Collagen type VI is a vital component in the extracellular matrix, contributing to tissue structure and function. Its dysregulation is associated with a range of diseases, including muscular dystrophy, fibrosis, and connective tissue disorders.
As a fundamental biomarker, studying collagen type VI can provide valuable insights into these conditions and aid in the development of therapeutic interventions.Overall, the Human COL6 (Collagen Type VI) ELISA Kit is a valuable tool for researchers seeking to explore the role of collagen type VI in health and disease, offering reliable and accurate measurement for in-depth investigations.
Product Name:
Human COL6 (Collagen Type VI) ELISA Kit
SKU:
HUES01913
Target:
Human COL6 (Collagen Type VI)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
4.69 ng/mL
Detection range:
7.81-500 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human COL6. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human COL6 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human COL6, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human COL6. You can calculate the concentration of Human COL6 in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
92-107
97-111
97-112
Average (%)
98
104
104
1:4
Range (%)
92-106
80-91
85-98
Average (%)
99
85
90
1:8
Range (%)
87-102
84-97
82-95
Average (%)
94
90
87
1:16
Range (%)
91-105
84-96
83-96
Average (%)
98
89
88
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
86-97
92
EDTA plasma (n=5)
91-106
98
Cell culture media (n=5)
89-102
94
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
24.63
77.18
172.25
23.55
75.5
172.95
Standard deviation
1.34
3.37
5.68
1.57
3.99
6.12
C V (%)
5.44
4.37
3.3
6.67
5.28
3.54
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human COL6 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human COL6 in samples. No significant cross-reactivity or interference between Human COL6 and analogues was observed.
