The Human CRT (Calreticulin) ELISA Kit is specifically designed for the precise measurement of calreticulin levels in human samples, including serum, plasma, and cell culture supernatants. With its high sensitivity and specificity, this kit ensures accurate and reproducible results, making it an ideal tool for various research purposes.Calreticulin is a multifunctional protein that plays a critical role in various cellular processes, including calcium homeostasis, protein folding, and immune response modulation. Dysregulation of calreticulin expression has been linked to numerous diseases, such as cancer, autoimmune disorders, and cardiovascular diseases, making it a valuable biomarker for studying these conditions and developing potential therapeutic interventions.Overall, the Human CRT (Calreticulin) ELISA Kit provides researchers with a reliable and efficient method for analyzing calreticulin levels in human samples, enabling deeper insights into its role in health and disease.
Product Name:
Human CRT (Calreticulin) ELISA Kit
SKU:
HUES01790
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Sandwich
Assay type:
Sandwich-ELISA
Assay time:
3 h 30 min
Sensitivity:
0.1 ng/mL
Detection range:
0.16-10 ng/mL
Reovery:
80%-120%
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to the target protein. Standards or samples are added to the micro ELISA plate wells and bind to the immobilized antibody. A biotinylated detection antibody specific to the target protein is then added, followed by Avidin-Horseradish Peroxidase (HRP) conjugate. Free components are washed away. The substrate solution is added to each well, resulting in a color change. Only wells containing the target protein, detection antibody, and HRP conjugate will develop a blue color. The reaction is terminated by the addition of stop solution, resulting in a yellow color. The optical density (OD) is measured at 450 nm ± 2 nm. The OD value is directly proportional to the concentration of the target protein in the sample and is determined using a standard curve.
