The Human CTSB (Cathepsin B) ELISA Kit is meticulously designed to facilitate the precise and quantitative detection of Cathepsin B levels in various human biological samples. Cathepsin B, a member of the papain-like cysteine protease family, performs crucial roles in intracellular protein degradation, extracellular matrix remodeling, and various physiological processes. By accurately quantifying Cathepsin B, researchers can gain valuable insights into its involvement in cellular homeostasis, disease pathogenesis, and therapeutic targets. Our ELISA kit offers exceptional sensitivity and specificity, ensuring robust and reproducible results vital for elucidating Cathepsin B's diverse functions. Crafted with stringent quality control measures, this kit delivers reliable performance and user-friendly protocols, making it an outstanding choice for cutting-edge research applications. Trust Assay Genie's Cathepsin B ELISA Kit for accurate and dependable quantification of this essential biomarker in your research endeavors.
Product Name:
Human CTSB (Cathepsin B) ELISA Kit
SKU:
AEES00160
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Sandwich
Assay type:
Sandwich-ELISA
Assay time:
3 h 30 min
Sensitivity:
1.88 ng/mL
Detection range:
3.13-200 ng/mL
Reovery:
80%-120%
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to the target protein. Standards or samples are added to the micro ELISA plate wells and bind to the immobilized antibody. A biotinylated detection antibody specific to the target protein is then added, followed by Avidin-Horseradish Peroxidase (HRP) conjugate. Free components are washed away. The substrate solution is added to each well, resulting in a color change. Only wells containing the target protein, detection antibody, and HRP conjugate will develop a blue color. The reaction is terminated by the addition of stop solution, resulting in a yellow color. The optical density (OD) is measured at 450 nm ± 2 nm. The OD value is directly proportional to the concentration of the target protein in the sample and is determined using a standard curve.
