Human DCXR Recombinant Protein (RPPB1561)
- SKU:
- RPPB1561
- Product Type:
- Recombinant Protein
- Species:
- Human
- Uniprot:
- Q7Z4W1
- Research Area:
- Enzymes
Description
| Product Name: | Human DCXR Recombinant Protein |
| Product Code: | RPPB1561 |
| Size: | 10µg |
| Species: | Human |
| Target: | DCXR |
| Synonyms: | DCR, HCR2, HCRII, KIDCR, P34H, SDR20C1, Dicarbonyl/L-Xylulose Reductase, EC=1.1.1.10, Carbonyl reductase II, Kidney dicarbonyl reductase, Sperm surface protein P34H. |
| Source: | Escherichia Coli |
| Physical Appearance: | Sterile filtered colorless solution. |
| Formulation: | The DCXR (0.5mg/ml) solution containing 20mM Tris-HClbuffer (pH 8.0), 1mM DTT, 20% glycerol and 50mM NaCl. |
| Stability: | Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles. |
| Purity: | Greater than 95.0% as determined by SDS-PAGE. |
| Amino Acid Sequence: | MGSSHHHHHH SSGLVPRGSH MELFLAGRRV LVTGAGKGIG RGTVQALHAT GARVVAVSRT QADLDSLVRE CPGIEPVCVD LGDWEATERA LGSVGPVDLL VNNAAVALLQ PFLEVTKEAF DRSFEVNLRA VIQVSQIVAR GLIARGVPGA IVNVSSQCSQ RAVTNHSVYC STKGALDMLT KVMALELGPH KIRVNAVNPT VVMTSMGQAT WSDPHKAKTM LNRIPLGKFA EVEHVVNAIL FLLSDRSGMT TGSTLPVEGG FWAC |
| Biological Activity: | Specific activity is > 1,800 pmol/min/ug and isdefined as the amount of enzyme that oxidize 1pmole of xylitol to L-xyluloseper minute at pH 10.0 at 37C. |
DCXR catalyzes the NADPH-dependent reduction of numerous pentoses, tetroses, trioses, alpha-dicarbonyl molecules and L-xylulose. DCXR takes part in the uronate cycle of glucose metabolism. DCXR participates in the water absorption andcellular osmoregulation in the proximal renal tubules by producing xylitol, an osmolyte, thus preventing osmolytic stress from occurring in the renal tubules.
DCXR Recombinant Human produced in E.Coli is a single, non-glycosylated polypeptide chain containing 264 amino acids (1-244 a.a.) and having a molecular mass of 28 kDa. The DCXR is fused to a 20 amino acids His-Tag at N-terminus and purified by proprietary chromatographic techniques.
| UniProt Protein Function: | DCXR: Catalyzes the NADPH-dependent reduction of several pentoses, tetroses, trioses, alpha-dicarbonyl compounds and L- xylulose. Participates in the uronate cycle of glucose metabolism. May play a role in the water absorption and cellular osmoregulation in the proximal renal tubules by producing xylitol, an osmolyte, thereby preventing osmolytic stress from occurring in the renal tubules. The enzyme defect in pentosuria has been shown to involve L-xylulose reductase. Essential pentosuria is an inborn error of metabolism characterized by the excessive urinary excretion of the pentose L-xylulose. Belongs to the short-chain dehydrogenases/reductases (SDR) family. |
| UniProt Protein Details: | Protein type:Oxidoreductase; Carbohydrate Metabolism - pentose and glucuronate interconversions; EC 1.1.1.10 Chromosomal Location of Human Ortholog: 17q25.3 Cellular Component: microvillus; membrane; cytoplasmic microtubule; nucleus; brush border Molecular Function:oxidoreductase activity, acting on NADH or NADPH, quinone or similar compound as acceptor; L-xylulose reductase activity Biological Process: glucose metabolic process; xylulose metabolic process; D-xylose metabolic process; NADP metabolic process; protein homotetramerization Disease: Pentosuria |
| NCBI Summary: | The protein encoded by this gene acts as a homotetramer to catalyze diacetyl reductase and L-xylulose reductase reactions. The encoded protein may play a role in the uronate cycle of glucose metabolism and in the cellular osmoregulation in the proximal renal tubules. Defects in this gene are a cause of pentosuria. Two transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Aug 2010] |
| UniProt Code: | Q7Z4W1 |
| NCBI GenInfo Identifier: | 50400451 |
| NCBI Gene ID: | 51181 |
| NCBI Accession: | Q7Z4W1.2 |
| UniProt Related Accession: | Q7Z4W1 |
| Molecular Weight: | |
| NCBI Full Name: | L-xylulose reductase |
| NCBI Synonym Full Names: | dicarbonyl and L-xylulose reductase |
| NCBI Official Symbol: | DCXR�� |
| NCBI Official Synonym Symbols: | XR; DCR; HCR2; P34H; HCRII; KIDCR; PNTSU; SDR20C1�� |
| NCBI Protein Information: | L-xylulose reductase |
| UniProt Protein Name: | L-xylulose reductase |
| UniProt Synonym Protein Names: | Carbonyl reductase II; Dicarbonyl/L-xylulose reductase; Kidney dicarbonyl reductase; kiDCR; Sperm surface protein P34H |
| UniProt Gene Name: | DCXR�� |
| UniProt Entry Name: | DCXR_HUMAN |
