The Human DEFB4A (Beta-Defensin 4A) ELISA Kit is a reliable and accurate tool for measuring DEFB4A levels in human samples such as serum, plasma, and cell culture supernatants. With high sensitivity and specificity, this kit ensures precise and reproducible results, making it suitable for a wide range of research applications. DEFB4A, also known as Beta-Defensin 4A, is a key component of the innate immune system and plays a crucial role in defending against microbial pathogens. Its expression is linked to various inflammatory conditions and diseases, making it a valuable biomarker for studying immune responses, infection, and inflammatory disorders.
By using the Human DEFB4A ELISA Kit, researchers can accurately quantify DEFB4A levels in biological samples, allowing for a better understanding of its role in health and disease. This kit provides a convenient and efficient means to study the function and regulation of DEFB4A, paving the way for new insights and potential therapeutic interventions.
Product Name:
Human DEFB4A/Beta-defensin 4A ELISA Kit
SKU:
HUFI00616
Reactivity:
Human
Assay Type:
Sandwich ELISA, Double Antibody
Sensitivity:
37.5 pg/mL
Range:
62.5-4000 pg/mL
Sample Type:
Serum, Plasma, Cell Culture Supernatant, Cell or Tissue Lysate, Other Liquid Samples
Storage:
2-8°C for 12 months.
Linearity:
Sample
1:2
1:4
1:8
Serum (n = 5)
88-105%
86-94%
93-104%
EDTA Plasma (n = 5)
86-97%
84-96%
83-99%
Heparin Plasma (n = 5)
80-97%
83-96%
80-86%
Recovery:
Sample
Recovery Range (%)
Average (%)
Serum (n = 5)
87-97
92
EDTA Plasma (n = 5)
86-103
91
Heparin Plasma (n = 5)
86-101
96
Note:The below protocol is a sample protocol. Protocols are specific to each batch/lot. For the correct instructions please follow the protocol included in your kit.
Step
Procedure
1
Reagent & Plate Preparation: Equilibrate reagents and TMB substrate to room temperature. Set standard, test sample and control (zero) wells on the pre-coated plate and record their positions.
2
Primary Incubation: Prepare standards, samples, blanks and load into designated wells. Incubate plate at 37°C for 90 minutes to allow antigen binding.
3
Detection Antibody Binding: Add biotin-labeled detection antibody and incubate at 37°C for 60 minutes.
4
HRP-Streptavidin Binding: Add HRP-Streptavidin (SABC) and incubate at 37°C for 30 minutes.
5
Color Development: Add TMB substrate and incubate in the dark for 10–20 minutes.
6
Stop Reaction & Reading: Add stop solution and measure absorbance at 450 nm immediately.
Sample Type
Protocol
Serum
Allow blood to clot, centrifuge at 1000 × g for 20 minutes, collect supernatant supernatant and store appropriately.
Plasma
Collect using anticoagulant tubes, centrifuge at 1000 × g for 15 minutes at 2–8°C and collect plasma.
Tissue Homogenate
Homogenize tissue in PBS with protease inhibitors, centrifuge and collect supernatant.
Cell Culture Supernatant
Centrifuge at 2500 rpm for 5 minutes and collect clarified supernatant.
Cell Lysate
Lyse cells using lysis buffer with protease inhibitors, centrifuge and collect protein supernatant.
Other Sample Types
For more information about how to process other sample types, (e.g., body fluids, breast milk & more), please contact our Tech Support Team at techsupport@assaygenie.com.
Component
Quantity
Storage
48T
96T
ELISA Microplate (Dismountable)
8×6
8×12
Place the test strips into a sealed foil bag with the desiccant. Store for 1 month at 2-8°C; Store for 12 months at -20°C.
Lyophilized Standard
1 vial
2 vial
Place the standards into a sealed foil bag with the desiccant. Store for 1 month at 2-8°C; Store for 12 months at -20°C.
