The Human E-cad (E-cadherin) ELISA Kit from Assay Genie is a reliable and accurate tool for detecting E-cadherin levels in human serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, ensuring precise and reproducible results for a variety of research applications.E-cadherin is a critical protein involved in cell adhesion and epithelial-mesenchymal transition, playing a crucial role in tissue organization and development. Aberrant E-cadherin expression is associated with various diseases, including cancer and metastasis, making it a valuable biomarker for studying these conditions and potential therapeutic interventions.
The Assay Genie Human E-cad ELISA Kit provides researchers with a powerful tool to accurately quantify E-cadherin levels, ultimately advancing our understanding of cellular processes and disease mechanisms. Upgrade your research with this high-quality ELISA kit from Assay Genie.
Product Name:
Human E-Cad (E-Cadherin) ELISA Kit
SKU:
HUES01301
Target:
Human E-Cad (E-Cadherin)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
0.10 ng/mL
Detection range:
0.16-10 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human E-Cad. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human E-Cad and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human E-Cad, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human E-Cad. You can calculate the concentration of Human E-Cad in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
92-103
92-105
85-99
Average (%)
98
97
91
1:4
Range (%)
101-116
84-97
96-112
Average (%)
107
90
102
1:8
Range (%)
101-112
84-99
91-107
Average (%)
106
90
98
1:16
Range (%)
95-110
85-100
98-112
Average (%)
101
91
105
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
88-105
96
EDTA plasma (n=5)
94-109
101
Cell culture media (n=5)
89-100
95
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
0.5
1.1
4.9
0.5
1.0
4.9
Standard deviation
0.03
0.05
0.15
0.03
0.04
0.15
C V (%)
6
4.55
3.06
6.0
4.0
3.06
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human E-Cad concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human E-Cad in samples. No significant cross-reactivity or interference between Human E-Cad and analogues was observed.
