The Human EGF (Epidermal Growth Factor) ELISA Kit is specifically designed to quantitatively measure EGF levels in human serum, plasma, and cell culture supernatants. With its high sensitivity and specificity, this kit provides accurate and reproducible results, making it a valuable tool for researchers in various fields.Epidermal Growth Factor (EGF) is a key protein that plays a vital role in cell growth, proliferation, and differentiation. It is known to be involved in various physiological processes, such as wound healing, tissue regeneration, and development. Dysregulation of EGF signaling has been linked to various diseases, including cancer and inflammatory disorders.By using the Human EGF ELISA Kit, researchers can gain valuable insights into EGF levels in biological samples, allowing for a better understanding of its role in health and disease. This kit is essential for studying EGF-related pathways and developing potential therapeutic interventions for EGF-associated conditions.
Product Name:
Human EGF (Epidermal Growth Factor) ELISA Kit
SKU:
HUES01346
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Sandwich
Assay type:
Sandwich-ELISA
Assay time:
3 h 30 min
Sensitivity:
2.35 pg/mL
Detection range:
3.91-250 pg/mL
Reovery:
80%-120%
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to the target protein. Standards or samples are added to the micro ELISA plate wells and bind to the immobilized antibody. A biotinylated detection antibody specific to the target protein is then added, followed by Avidin-Horseradish Peroxidase (HRP) conjugate. Free components are washed away. The substrate solution is added to each well, resulting in a color change. Only wells containing the target protein, detection antibody, and HRP conjugate will develop a blue color. The reaction is terminated by the addition of stop solution, resulting in a yellow color. The optical density (OD) is measured at 450 nm ± 2 nm. The OD value is directly proportional to the concentration of the target protein in the sample and is determined using a standard curve.
