The Human ELN (Elastin) ELISA Kit is a powerful tool for researchers looking to accurately measure elastin levels in human samples such as serum, plasma, and cell culture supernatants. With its high sensitivity and specificity, this kit guarantees precise and reliable results, suitable for a variety of research applications.Elastin is a key protein responsible for providing elasticity to tissues in the body, particularly in blood vessels and skin. Changes in elastin levels have been linked to various health conditions, including cardiovascular diseases and skin disorders. Therefore, measuring elastin levels using this ELISA kit can provide valuable insights into disease mechanisms and potential therapeutic strategies.Whether investigating the role of elastin in tissue repair, aging, or disease progression, the Human ELN ELISA Kit from Assay Genie is a valuable tool for researchers seeking to deepen their understanding of elastin biology and its implications for human health.
Product Name:
Human ELN (Elastin) ELISA Kit
SKU:
HUES02243
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Sandwich
Assay type:
Sandwich-ELISA
Assay time:
3 h 30 min
Sensitivity:
0.28 ng/mL
Detection range:
0.47-30 ng/mL
Reovery:
80%-120%
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to the target protein. Standards or samples are added to the micro ELISA plate wells and bind to the immobilized antibody. A biotinylated detection antibody specific to the target protein is then added, followed by Avidin-Horseradish Peroxidase (HRP) conjugate. Free components are washed away. The substrate solution is added to each well, resulting in a color change. Only wells containing the target protein, detection antibody, and HRP conjugate will develop a blue color. The reaction is terminated by the addition of stop solution, resulting in a yellow color. The optical density (OD) is measured at 450 nm ± 2 nm. The OD value is directly proportional to the concentration of the target protein in the sample and is determined using a standard curve.
