The Human ET-1 (Endothelin-1) ELISA Kit is specifically designed for the precise quantitative measurement of Endothelin-1 levels in human serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, guaranteeing accurate and reproducible results for various research applications.Endothelin-1 is a vital peptide involved in vasoconstriction and regulation of blood pressure, making it a key player in cardiovascular diseases and hypertension.
Its dysregulation has been linked to conditions such as pulmonary hypertension, heart failure, and kidney diseases, highlighting its importance as a potential biomarker for disease diagnosis and monitoring.Overall, the Human ET-1 ELISA Kit provides researchers with a valuable tool for investigating the role of Endothelin-1 in various physiological and pathological processes, ultimately contributing to the development of innovative therapeutic strategies.
Product Name:
Human ET-1 (Endothelin 1) ELISA Kit
SKU:
HUES01351
Target:
Human ET-1 (Endothelin 1)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
0.75 pg/mL
Detection range:
1.25-80 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human ET-1. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human ET-1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human ET-1, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human ET-1. You can calculate the concentration of Human ET-1 in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
89-104
86-97
93-110
Average (%)
95
91
100
1:4
Range (%)
95-110
87-97
92-104
Average (%)
101
92
99
1:8
Range (%)
95-105
84-95
96-111
Average (%)
100
89
103
1:16
Range (%)
99-115
82-93
96-109
Average (%)
105
88
102
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
92-103
97
EDTA plasma (n=5)
89-104
96
Cell culture media (n=5)
86-100
92
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
4
7.8
33.6
4.0
7.7
36.3
Standard deviation
0.3
0.4
1.4
0.2
0.3
1.4
C V (%)
7.5
5.13
4.17
5.0
3.9
3.86
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human ET-1 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human ET-1 in samples. No significant cross-reactivity or interference between Human ET-1 and analogues was observed.
