The Human FN (Fibronectin) ELISA Kit is specifically created for the precise measurement of fibronectin levels in human serum, plasma, and cell culture supernatants. With its high sensitivity and specificity, this kit ensures dependable and consistent results, making it suitable for a diverse array of research purposes.Fibronectin is an essential glycoprotein that plays a key role in cell adhesion, growth, migration, and differentiation. It is involved in various physiological processes such as wound healing, embryonic development, and tissue repair.
Abnormal fibronectin levels are associated with conditions like cancer, fibrosis, and inflammatory diseases, highlighting its importance as a biomarker for investigating these disorders and potential therapeutic interventions.Get accurate and reliable results with the Human FN (Fibronectin) ELISA Kit, and unlock new insights into the role of fibronectin in health and disease.
Product Name:
Human FN (Fibronectin) ELISA Kit
SKU:
HUES01446
Target:
Human FN (Fibronectin)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
0.94 ng/mL
Detection range:
1.56-100 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human FN. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human FN and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human FN, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human FN. You can calculate the concentration of Human FN in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
92-108
93-109
91-104
Average (%)
99
99
98
1:4
Range (%)
86-102
86-99
82-96
Average (%)
93
91
89
1:8
Range (%)
92-107
87-98
89-103
Average (%)
98
92
94
1:16
Range (%)
88-100
85-97
84-94
Average (%)
93
91
89
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
89-102
96
EDTA plasma (n=5)
94-110
101
Cell culture media (n=5)
91-105
98
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
5.02
14.93
40.64
4.58
15.48
37.14
Standard deviation
0.29
0.67
1.38
0.24
0.64
1.15
C V (%)
5.78
4.49
3.4
5.24
4.13
3.1
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human FN concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human FN in samples. No significant cross-reactivity or interference between Human FN and analogues was observed.
