The Human GAL7 (Galectin-7) CLIA Kit is a highly sensitive and specific assay designed for the accurate detection of galectin-7 levels in human samples such as serum, plasma, and cell culture supernatants. Galectin-7 is a key protein involved in regulating cell growth, apoptosis, and inflammation, making it a valuable biomarker for various disease conditions.This kit provides researchers with reliable and reproducible results, making it ideal for a wide range of applications in the field of biomedicine and diagnostics.
Galectin-7 has been implicated in various diseases such as cancer, inflammatory disorders, and autoimmune diseases, highlighting its potential as a therapeutic target.With its high sensitivity and specificity, the Human GAL7 CLIA Kit offers a powerful tool for studying the role of galectin-7 in disease pathology and for developing novel treatment strategies. Unlock the potential of galectin-7 research with this advanced CLIA kit from Assay Genie.
Product Name:
Human GAL7 (Galectin 7) CLIA Kit
SKU:
HUES00623
Target:
Human GAL7 (Galectin 7)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human GAL7. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human GAL7 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human GAL7, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human GAL7. You can calculate the concentration of Human GAL7 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
99-113
90-106
98-114
Average (%)
106
97
105
1:4
Range (%)
101-117
87-100
87-97
Average (%)
109
93
92
1:8
Range (%)
96-111
98-114
103-116
Average (%)
102
106
110
1:16
Range (%)
87-101
95-110
95-108
Average (%)
94
101
101
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
89-102
95
EDTA plasma (n=5)
97-113
105
Cell culture media (n=5)
85-98
92
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
106.37
200.68
875.29
103.33
195.52
902.98
Standard deviation
13.03
23.64
81.66
10.09
22.37
102.67
C V (%)
12.25
11.78
9.33
9.76
11.44
11.37
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human GAL7 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human GAL7 in samples. No significant cross-reactivity or interference between Human GAL7 and analogues was observed.
