Human GCA Recombinant Protein (RPPB3581)
- SKU:
- RPPB3581
- Product Type:
- Recombinant Protein
- Species:
- Human
- Uniprot:
- P28676
Description
| Product Name: | Human GCA Recombinant Protein |
| Product Code: | RPPB3581 |
| Size: | 10µg |
| Species: | Human |
| Target: | GCA |
| Synonyms: | Grancalcin EF-hand calcium binding protein, GCL, Grancalcin penta-EF-hand protein. |
| Source: | Escherichia Coli |
| Physical Appearance: | Sterile Filtered clear solution. |
| Formulation: | The GCA protein solution (1mg/1ml) is formulated in 20mM Tris-HCl buffer (pH8.0) 0.1M NaCl and 20% glycerol. |
| Stability: | Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time.Please avoid freeze thaw cycles. |
| Purity: | Greater than 95% as determined by SDS-PAGE. |
| Amino Acid Sequence: | MGSSHHHHHH SSGLVPRGSH MAYPGYGGGF GNFSIQVPGM QMGQPVPETG PAILLDGYSG PAYSDTYSSA GDSVYTYFSA VAGQDGEVDA EELQRCLTQS GINGTYSPFS LETCRIMIAM LDRDHTGKMG FNAFKELWAA LNAWKENFMT VDQDGSGTVE HHELRQAIGL MGYRLSPQTL TTIVKRYSKN GRIFFDDYVA CCVKLRALTD FFRKRDHLQQ GSANFIYDDF LQGTMAI |
Grancalcin is calcium-binding protein which is particularly abundant in human neutrophils. GCA is a member of the penta EF-hand (PEF) subfamily of EF-hand proteins, who also comprises calpain, sorcin, peflin, and ALG-2. GCA undergoes essential conformational changes upon binding of calcium, which subsequently exposes hydrophobic amino acid residues, that direct the protein to hydrophobic surfaces. GCA cooperates with L-plastin, a protein known to have actin bundling activity, which suggests that GCA has a part in the regulation of neutrophils adhesion.
GCA produced in E.Coli is a single, non-glycosylated polypeptide chain containing 237 amino acids (1-217.a.) and having a molecular mass of 26.1kDa. GCA is fused to a 20 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
| UniProt Protein Function: | GCA: Calcium-binding protein that may play a role in the adhesion of neutrophils to fibronectin. May play a role in the formation of focal adhesions. |
| UniProt Protein Details: | Protein type:Calcium-binding Chromosomal Location of Human Ortholog: 2q24.2 Cellular Component: cytoplasm; plasma membrane Molecular Function:protein homodimerization activity; protein heterodimerization activity; calcium-dependent cysteine-type endopeptidase activity; calcium ion binding Biological Process: proteolysis |
| NCBI Summary: | This gene product, grancalcin, is a calcium-binding protein abundant in neutrophils and macrophages. It belongs to the penta-EF-hand subfamily of proteins which includes sorcin, calpain, and ALG-2. Grancalcin localization is dependent upon calcium and magnesium. In the absence of divalent cation, grancalcin localizes to the cytosolic fraction; with magnesium alone, it partitions with the granule fraction; and in the presence of magnesium and calcium, it associates with both the granule and membrane fractions, suggesting a role for grancalcin in granule-membrane fusion and degranulation. [provided by RefSeq, Jul 2008] |
| UniProt Code: | P28676 |
| NCBI GenInfo Identifier: | 1170014 |
| NCBI Gene ID: | 25801 |
| NCBI Accession: | P28676.2 |
| UniProt Secondary Accession: | P28676,Q53TB5, Q59EP3, B2R5X3, |
| UniProt Related Accession: | P28676 |
| Molecular Weight: | 217 |
| NCBI Full Name: | Grancalcin |
| NCBI Synonym Full Names: | grancalcin, EF-hand calcium binding protein |
| NCBI Official Symbol: | GCA�� |
| NCBI Official Synonym Symbols: | GCL�� |
| NCBI Protein Information: | grancalcin; grancalcin, penta-EF-hand protein; grancalcin, EF-hand calcium-binding protein |
| UniProt Protein Name: | Grancalcin |
| Protein Family: | Grancalcin |
| UniProt Gene Name: | GCA�� |
| UniProt Entry Name: | GRAN_HUMAN |
