Human GDNF (Glial Cell Line Derived Neurotrophic Factor) CLIA Kit
The Human GDNF (Glial Cell Line-Derived Neurotrophic Factor) CLIA Kit is designed for the precise measurement of GDNF levels in human samples, including serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, ensuring accurate and reproducible results for a variety of research purposes.GDNF is a critical neurotrophic factor that supports the survival and function of neurons in the central and peripheral nervous systems. Abnormalities in GDNF levels have been associated with various neurological disorders, including Parkinson's disease and amyotrophic lateral sclerosis (ALS).
As such, GDNF serves as a valuable biomarker for studying these conditions and developing potential therapeutic interventions.With its advanced technology and reliable performance, the Human GDNF CLIA Kit is an indispensable tool for researchers investigating the role of GDNF in neurological health and disease. Get your kit today and unlock new insights into the complex mechanisms underlying neurodegenerative disorders.
Product Name:
Human GDNF (Glial Cell Line Derived Neurotrophic Factor) CLIA Kit
SKU:
HUES00851
Target:
Human GDNF (Glial Cell Line Derived Neurotrophic Factor)
Size:
96T
Assay type:
Sandwich-CLIA
Assay time:
3.5h
Sensitivity:
9.38 pg/mL
Detection range:
15.63-1000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human GDNF. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human GDNF and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human GDNF, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human GDNF. You can calculate the concentration of Human GDNF in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
87-97
96-111
88-101
Average (%)
92
103
93
1:4
Range (%)
102-119
99-114
84-96
Average (%)
109
108
91
1:8
Range (%)
87-101
92-108
100-112
Average (%)
94
100
106
1:16
Range (%)
98-114
86-101
88-99
Average (%)
105
92
93
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
86-101
93
EDTA plasma (n=5)
91-105
96
Cell culture media (n=5)
88-100
94
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
48.89
111.48
476.63
53.07
112.07
448.71
Standard deviation
5.55
7.99
32.03
5.58
11.99
49.99
C V (%)
11.35
7.17
6.72
10.51
10.7
11.14
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human GDNF concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human GDNF in samples. No significant cross-reactivity or interference between Human GDNF and analogues was observed.
