Human GFAP (Glial Fibrillary Acidic Protein) ELISA Kit
The Human GFAP (Glial Fibrillary Acidic Protein) ELISA Kit is expertly designed to quantitatively detect levels of GFAP in a variety of human biological samples. GFAP, a vital protein predominantly found in astrocytes, serves as a marker for astrocytic activation and is instrumental in maintaining central nervous system integrity. Its quantification plays a crucial role in understanding neuroinflammatory processes, neural injury, and diseases involving astrocyte dysfunction. Our GFAP ELISA Kit ensures exceptional sensitivity and specificity, guaranteeing accurate and reproducible results. With stringent manufacturing processes and quality control measures, this kit offers robust performance and user-friendly procedures, making it an excellent choice for both research and clinical purposes. Rely on our GFAP ELISA Kit for precise and reliable quantification of this significant biomarker in your scientific endeavors.
Product Name:
Human GFAP (Glial Fibrillary Acidic Protein) ELISA Kit
SKU:
AEES00127
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Sandwich
Assay type:
Sandwich-ELISA
Assay time:
3 h 30 min
Sensitivity:
9.38 pg/mL
Detection range:
15.63-1000 pg/mL
Reovery:
80%-120%
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to the target protein. Standards or samples are added to the micro ELISA plate wells and bind to the immobilized antibody. A biotinylated detection antibody specific to the target protein is then added, followed by Avidin-Horseradish Peroxidase (HRP) conjugate. Free components are washed away. The substrate solution is added to each well, resulting in a color change. Only wells containing the target protein, detection antibody, and HRP conjugate will develop a blue color. The reaction is terminated by the addition of stop solution, resulting in a yellow color. The optical density (OD) is measured at 450 nm ± 2 nm. The OD value is directly proportional to the concentration of the target protein in the sample and is determined using a standard curve.
