Human GLRX2 Recombinant Protein (RPPB1701)
- SKU:
- RPPB1701
- Product Type:
- Recombinant Protein
- Species:
- Human
- Uniprot:
- Q9NS18
- Research Area:
- Enzymes
Description
| Product Name: | Human GLRX2 Recombinant Protein |
| Product Code: | RPPB1701 |
| Size: | 25µg |
| Species: | Human |
| Target: | GLRX2 |
| Synonyms: | Thioltransferase, Glutathione-dependent oxidoreductase 2, TTR, TTR1, GLRX2, GRX2, GRX-2, GLRX-2, Glutaredoxin 2,�CGI133. |
| Source: | Escherichia Coli |
| Physical Appearance: | Sterile Filtered clear colorless solution. |
| Formulation: | Glutaredoxin-2 solution (0.5mg/ml) contains 20mM Tris-HCl Buffer (pH 8.0), 0.1mM PMSF and 10% glycerol. |
| Stability: | Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles. |
| Purity: | Purity of GRX2 is greater than 90% as determined by SDS-PAGE. |
| Amino Acid Sequence: | MSAGWLDRAA GAAGAAAAAA SGMESNTSSS LENLATAPVN QIQETISDNC VVIFSKTSCS YCTMAKKLFH DMNVNYKVVE LDLLEYGNQF QDALYKMTGE RTVPRIFVNG TFIGGATDTH RLHKEGKLLP LVHQCYLKKS KRKEFQLEHH HHHH |
GLRX2 is a multifunctional enzyme with glutathione-dependent oxidoreductase, glutathione peroxidase and glutathione S-transferase (GST) activity. The disulfide bond functions as an electron carrier in the glutathione-dependent synthesis of deoxyribonucleotides by the enzyme ribonucleotide reductase. In addition, it is also involved in reducing cytosolic protein- and non-protein-disulfides in a coupled system with glutathione reductase. Required for resistance to reactive oxygen species (ROS) by directly reducing hydroperoxides and for the detoxification of ROS-mediated damage. Glutaredoxins are a family of glutathione-dependent hydrogen donors that participate in a variety of cellular redox reactions.
Glutaredoxin-2 Human Recombinant produced in E.Coli is a single, non-glycosylated, Polypeptide chain containing 154 amino acids (20-164 a.a.) and having a molecular mass of 17 kDa. The GRX2 is fused to�9 amino acid His tag at C-Terminus.
| UniProt Protein Function: | GLRX2: Glutathione-dependent oxidoreductase that facilitates the maintenance of mitochondrial redox homeostasis upon induction of apoptosis by oxidative stress. Involved in response to hydrogen peroxide and regulation of apoptosis caused by oxidative stress. Acts as a very efficient catalyst of monothiol reactions because of its high affinity for protein glutathione-mixed disulfides. Can receive electrons not only from glutathione (GSH), but also from thioredoxin reductase supporting both monothiol and dithiol reactions. Efficiently catalyzes both glutathionylation and deglutathionylation of mitochondrial complex I, which in turn regulates the superoxide production by the complex. Overexpression decreases the susceptibility to apoptosis and prevents loss of cardiolipin and cytochrome c release. Belongs to the glutaredoxin family. 2 isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Protein type:Oxidoreductase; Mitochondrial Chromosomal Location of Human Ortholog: 1q31.2 Cellular Component: nucleoplasm; mitochondrion; intracellular membrane-bound organelle; nucleus Molecular Function:2 iron, 2 sulfur cluster binding; electron carrier activity; metal ion binding; protein disulfide oxidoreductase activity; protein disulfide isomerase activity; arsenate reductase (glutaredoxin) activity; glutathione disulfide oxidoreductase activity Biological Process: response to organic substance; response to temperature stimulus; DNA protection; glutathione metabolic process; response to hydrogen peroxide; regulation of transcription, DNA-dependent; protein folding; apoptosis; cell redox homeostasis; regulation of signal transduction; response to redox state; cell differentiation |
| NCBI Summary: | The protein encoded by this gene is a member of the glutaredoxin family of proteins, which maintain cellular thiol homeostasis. These proteins are thiol-disulfide oxidoreductases that use a glutathione-binding site and one or two active cysteines in their active site. This gene undergoes alternative splicing to produce multiple isoforms, one of which is ubiquitously expressed and localizes to mitochondria, where it functions in mitochondrial redox homeostasis and is important for the protection against and recovery from oxidative stress. Other isoforms, which have more restrictive expression patterns, show cytosolic and nuclear localization, and are thought to function in cellular differentiation and transformation, possibly with a role in tumor progression. [provided by RefSeq, Aug 2011] |
| UniProt Code: | Q9NS18 |
| NCBI GenInfo Identifier: | 73919686 |
| NCBI Gene ID: | 51022 |
| NCBI Accession: | Q9NS18.1 |
| UniProt Secondary Accession: | Q9NS18,Q3LR69, Q7L1N7, Q96JC0, Q9Y3D4, |
| UniProt Related Accession: | Q9NS18 |
| Molecular Weight: | 18,726 Da |
| NCBI Full Name: | Glutaredoxin-2, mitochondrial |
| NCBI Synonym Full Names: | glutaredoxin 2 |
| NCBI Official Symbol: | GLRX2�� |
| NCBI Official Synonym Symbols: | GRX2; CGI-133�� |
| NCBI Protein Information: | glutaredoxin 2; bA101E13.1 (GRX2 glutaredoxin (thioltransferase) 2) |
| UniProt Protein Name: | Glutaredoxin-2, mitochondrial |
| UniProt Gene Name: | GLRX2�� |
| UniProt Entry Name: | GLRX2_HUMAN |
