Human GNAI1 Recombinant Protein (RPPB5888)
- SKU:
- RPPB5888
- Product Type:
- Recombinant Protein
- Species:
- Human
- Uniprot:
- P63096
Description
| Product Name: | Human GNAI1 Recombinant Protein |
| Product Code: | RPPB5888 |
| Size: | 10µg |
| Species: | Human |
| Target: | GNAI1 |
| Synonyms: | Guanine nucleotide-binding protein G(i) subunit alpha-1, Adenylate cyclase-inhibiting G alpha protein, GNAI1, Gi. |
| Source: | Escherichia Coli |
| Physical Appearance: | Sterile Filtered colorless solution. |
| Formulation: | GNAI1 protein solution (1mg/ml) containing 20mM Tris-HCl buffer (pH8.0) and 10% glycerol. |
| Stability: | Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles. |
| Purity: | Greater than 90.0% as determined by SDS-PAGE. |
| Amino Acid Sequence: | MGSSHHHHHH SSGLVPRGSH MGSMGCTLSA EDKAAVERSK MIDRNLREDG EKAAREVKLL LLGAGESGKS TIVKQMKIIH EAGYSEEECK QYKAVVYSNT IQSIIAIIRA MGRLKIDFGD SARADDARQL FVLAGAAEEG FMTAELAGVI KRLWKDSGVQ ACFNRSREYQ LNDSAAYYLN DLDRIAQPNY IPTQQDVLRT RVKTTGIVET HFTFKDLHFK MFDVGGQRSE RKKWIHCFEG VTAIIFCVAL SDYDLVLAED EEMNRMHESM KLFDSICNNK WFTDTSIILF LNKKDLFEEK IKKSPLTICY PEYAGSNTYE EAAAYIQCQF EDLNKRKDTK EIYTHFTCAT DTKNVQFVFD AVTDVIIKNN LKDCGLF |
Guanine nucleotide binding proteins are heterotrimeric signal-transducing molecules comprising alpha, beta, and gamma subunits. GNAI1 represents the alpha subunit of an inhibitory complex. Guanine nucleotide-binding protein G(i) subunit alpha (GNAI1) functions to transmit information from cell surface receptors to intracellular effectors. GNAI1 binds guanine nucleotide, can hydrolyze GTP, and can interact with other proteins. GNAI1 is part of a complex that responds to beta-adrenergic signals by inhibiting adenylate cyclase. In addition, GNAI1 functions to open atrial potassium channels.
GNAI1 Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 377 amino acids (1-354 a.a.) and having a molecular mass of 42.7kDa.GNAI1 is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
| UniProt Protein Function: | G-alpha i1: Guanine nucleotide-binding proteins (G proteins) are involved as modulators or transducers in various transmembrane signaling systems. The G(i) proteins are involved in hormonal regulation of adenylate cyclase: they inhibit the cyclase in response to beta-adrenergic stimuli. The inactive GDP-bound form prevents the association of RGS14 with centrosomes and is required for the translocation of RGS14 from the cytoplasm to the plasma membrane. May play a role in cell division. Interacts with GPSM1. Interacts with RGS12. Interacts (via active GTP- or inactive GDP-bound forms) with RGS14 (via RGS and GoLoco domains); the interaction occurs in the centrosomes. Interacts (GDP-bound form) with RIC8A (via C-terminus). Interacts with DRD2. G proteins are composed of 3 units; alpha, beta and gamma. The alpha chain contains the guanine nucleotide binding site. Belongs to the G-alpha family. G(i/o/t/z) subfamily. |
| UniProt Protein Details: | Protein type:G protein, heterotrimeric; G protein; G protein, heterotrimeric alpha G((i/o/t/z)) Chromosomal Location of Human Ortholog: 7q21 Cellular Component: centrosome; lysosomal membrane; cytoplasm; plasma membrane; heterotrimeric G-protein complex; midbody; nucleus Molecular Function:GTPase activity; protein binding; signal transducer activity; GTP binding; metal ion binding; G-protein beta/gamma-subunit binding; metabotropic serotonin receptor binding Biological Process: G-protein signaling, coupled to cAMP nucleotide second messenger; synaptic transmission; G-protein coupled receptor protein signaling pathway; response to peptide hormone stimulus; platelet activation; metabolic process; cell division; G-protein signaling, adenylate cyclase inhibiting pathway; blood coagulation; cell cycle |
| NCBI Summary: | Guanine nucleotide binding proteins are heterotrimeric signal-transducing molecules consisting of alpha, beta, and gamma subunits. The alpha subunit binds guanine nucleotide, can hydrolyze GTP, and can interact with other proteins. The protein encoded by this gene represents the alpha subunit of an inhibitory complex. The encoded protein is part of a complex that responds to beta-adrenergic signals by inhibiting adenylate cyclase. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jan 2012] |
| UniProt Code: | P63096 |
| NCBI GenInfo Identifier: | 52000964 |
| NCBI Gene ID: | 2770 |
| NCBI Accession: | P63096.2 |
| UniProt Secondary Accession: | P63096,P04898, P11015, P31871, Q5U074, Q8TAN5, Q9UGA4 A8KA88, B4E2V1, C9J3A4, |
| UniProt Related Accession: | P63096 |
| Molecular Weight: | 34,775 Da |
| NCBI Full Name: | Guanine nucleotide-binding protein G(i) subunit alpha-1 |
| NCBI Synonym Full Names: | guanine nucleotide binding protein (G protein), alpha inhibiting activity polypeptide 1 |
| NCBI Official Symbol: | GNAI1�� |
| NCBI Official Synonym Symbols: | Gi�� |
| NCBI Protein Information: | guanine nucleotide-binding protein G(i) subunit alpha-1; guanine nucleotide-binding protein G(i) subunit alpha-1; Gi1 protein alpha subunit; adenylate cyclase-inhibiting G alpha protein |
| UniProt Protein Name: | Guanine nucleotide-binding protein G(i) subunit alpha-1 |
| UniProt Synonym Protein Names: | Adenylate cyclase-inhibiting G alpha protein |
| UniProt Gene Name: | GNAI1�� |
| UniProt Entry Name: | GNAI1_HUMAN |
