Human GPNMB Recombinant Protein (RPPB3638)
- SKU:
- RPPB3638
- Product Type:
- Recombinant Protein
- Species:
- Human
- Uniprot:
- Q14956
Description
| Product Name: | Human GPNMB Recombinant Protein |
| Product Code: | RPPB3638 |
| Size: | 10µg |
| Species: | Human |
| Target: | GPNMB |
| Synonyms: | Transmembrane glycoprotein NMB, Transmembrane glycoprotein HGFIN, GPNMB, HGFIN, NMB. |
| Source: | HEK293 Cells |
| Physical Appearance: | Filtered White lyophilized (freeze-dried) powder. |
| Formulation: | Filtered (0.4�m) and lyophilized from 0.5mg/ml in 0.05M phosphate buffer and 0.075M NaCl, pH 7.4. |
| Solubility: | It is recommended to add deionized water to a working concentration of 0.5mg/ml and let the lyophilized pellet dissolve completely. GPNMB is not sterile! Please filter the product by an appropriate sterile filter before using it in the cell culture. |
| Stability: | Store lyophilized protein at -20°C. Aliquot the product after reconstitution to avoid repeated freezing/thawing cycles. Reconstituted protein can be stored at 4°C for a limited period of time. |
| Purity: | Greater than 95.0% as determined by SDS-PAGE. |
| Amino Acid Sequence: | AKRFHDVLGN ERPSAYMREH NQLNGWSSDE NDWNEKLYPV WKRGDMRWKN SWKGGRVQAV LTSDSPALVG SNITFAVNLI FPRCQKEDAN GNIVYEKNCR NEAGLSADPY VYNWTAWSED SDGENGTGQS HHNVFPDGKP FPHHPGWRRW NFIYVFHTLG QYFQKLGRCS VRVSVNTANV TLGPQLMEVT VYRRHGRAYV PIAQVKDVYV VTDQIPVFVT MFQKNDRNSS DETFLKDLPI MFDVLIHDPS HFLNYSTINY KWSFGDNTGL FVSTNHTVNH TYVLNGTFSL NLTVKAAAPG PCPPPPPPPR PSKPTPSLAT TLKSYDSNTP GPAGDNPLEL SRIPDENCQI NRYGHFQATI TIVEGILEVN IIQMTDVLMP VPWPESSLID FVVTCQGSIP TEVCTIISDP TCEITQNTVC SPVDVDEMCL LTVRRTFNGS GTYCVNLTLG DDTSLALTST LISVP KLHHH HHHHHHH |
Glycoprotein Nmb (GPNMB) is a member of the PMEL/NMB family. GPNMB is a type I transmembrane glycoprotein which exhibits homology to the pMEL17 precursor, a melanocyte-specific protein. GPNMB is expressed in the lowly metastatic human melanoma cell lines and xenografts but has no expression in the highly metastatic cell lines. GPNMB might be involved in growth delay and reduction of metastatic potential. GPNMB is up-regulated in a number of cancer cells, including in glioblastoma multiforme. GPNMB is expressed in many melanoma cells, as well as in tissue macrophages, including liver Kuppfer cells and lung alveolar macrophages, in podocytes and in some cells of the ciliary body of the eye (at protein level). GPNMB is hardly detectable in the healthy brain.
GPNMB Human Recombinant produced in HEK cells is a single, glycosylated, polypeptide chain (a.a 22-486) containing a total of 477 amino acids, having a molecular mass of 53.7kDa (calculated), though it migrates at approximately 97kDa on SDS PAGE, the GPNMB is fused to a 2 a.a C-terminal linker and a 10 a.a His tag at C-Terminus.The Human GPNMB is purified by proprietary chromatographic techniques.
| UniProt Protein Function: | GPNMB: Could be a melanogenic enzyme. Belongs to the PMEL/NMB family. 2 isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Protein type:Membrane protein, integral Chromosomal Location of Human Ortholog: 7p15 Cellular Component: integral to plasma membrane; melanosome; integral to membrane Molecular Function:integrin binding; heparin binding Biological Process: osteoblast differentiation; negative regulation of cell proliferation; cell adhesion; bone mineralization |
| NCBI Summary: | The protein encoded by this gene is a type I transmembrane glycoprotein which shows homology to the pMEL17 precursor, a melanocyte-specific protein. GPNMB shows expression in the lowly metastatic human melanoma cell lines and xenografts but does not show expression in the highly metastatic cell lines. GPNMB may be involved in growth delay and reduction of metastatic potential. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008] |
| UniProt Code: | Q14956 |
| NCBI GenInfo Identifier: | 61252504 |
| NCBI Gene ID: | 10457 |
| NCBI Accession: | Q14956.2 |
| UniProt Secondary Accession: | Q14956,Q6UVX1, Q8N1A1, A4D155, |
| UniProt Related Accession: | Q14956 |
| Molecular Weight: | 62,643 Da |
| NCBI Full Name: | Transmembrane glycoprotein NMB |
| NCBI Synonym Full Names: | glycoprotein (transmembrane) nmb |
| NCBI Official Symbol: | GPNMB�� |
| NCBI Official Synonym Symbols: | NMB; HGFIN�� |
| NCBI Protein Information: | transmembrane glycoprotein NMB; osteoactivin; glycoprotein nmb-like protein; transmembrane glycoprotein HGFIN |
| UniProt Protein Name: | Transmembrane glycoprotein NMB |
| UniProt Synonym Protein Names: | Transmembrane glycoprotein HGFIN |
| Protein Family: | Transmembrane glycoprotein |
| UniProt Gene Name: | GPNMB�� |
| UniProt Entry Name: | GPNMB_HUMAN |
