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Human GSR Recombinant Protein (RPPB1749)

SKU:
RPPB1749
Product Type:
Recombinant Protein
Species:
Human
Uniprot:
P00390
Research Area:
Enzymes
€309
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Description

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Product Name:Human GSR Recombinant Protein
Product Code:RPPB1749
Size:10µg
Species:Human
Target:GSR
Synonyms:Glutathione reductase mitochondrial, GR, GRase, GSR, GLUR, GRD1.
Source:Escherichia Coli
Physical Appearance:Sterile Filtered colorless solution.
Formulation:The GSR solution (1mg/ml) contains 20mM Tris-HCl buffer (pH 8.0), 1mM DTT, 10% glycerol and 0.1M NaCl.
Stability:Store at 4°C if entire vial will be used within 2-4 weeks.Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.
Purity:Greater than 95.0% as determined by SDS-PAGE.
Amino Acid Sequence:MGSSHHHHHH SSGLVPRGSH MGSMAMACRQ EPQPQGPPPA AGAVASYDYL VIGGGSGGLA SARRAAELGA RAAVVESHKL GGTCVNVGCV PKKVMWNTAV HSEFMHDHAD YGFPSCEGKF NWRVIKEKRD AYVSRLNAIY QNNLTKSHIE IIRGHAAFTS DPKPTIEVSG KKYTAPHILI ATGGMPSTPH ESQIPGASLG ITSDGFFQLE ELPGRSVIVG AGYIAVEMAG ILSALGSKTS LMIRHDKVLR SFDSMISTNC TEELENAGVE VLKFSQVKEV KKTLSGLEVS MVTAVPGRLP VMTMIPDVDC LLWAIGRVPN TKDLSLNKLG IQTDDKGHII VDEFQNTNVK GIYAVGDVCG KALLTPVAIA AGRKLAHRLF EYKEDSKLDY NNIPTVVFSH PPIGTVGLTE DEAIHKYGIE NVKTYSTSFT PMYHAVTKRK TKCVMKMVCA NKEEKVVGIH MQGLGCDEML QGFAVAVKMG ATKADFDNTV AIHPTSSEEL VTLR
Biological Activity:Specific activity: > 29 unit/ml. One unit will reduce 1.0 umol of oxidized glutathione per minute at pH 7.5 at 25�C.

Glutathione reductase (GSR) belongs to the class-I pyridine nucleotide-disulfide oxidoreductase family. The GSR enzyme is a homodimeric flavoprotein and has a role in maintaining glutathione (GSH) in its reduced form by catalyzing the reduction of glutathione disulfide (GSSG): GSSG + NADPH + H+ ->2GSH + NADP+. In the majority of eukaryotic cells, GSR upholds the ratio of [GSH] / [GSSG], and partakes in quite a few critical functions such as the detoxification of reactive oxygen species as well as protein and DNA biosynthesis.

GSR Human Recombinant produced in E.coli is a single, non-glycosylated polypeptide chain containing 504 amino acids (43-522) and having a molecular mass of 54.3kDa.GSR is fused to a 24 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

UniProt Protein Function:GSR: a class-I pyridine nucleotide-disulfide oxidoreductase. Maintains high levels of reduced glutathione in the cytosol. Alternative initiation results in a mitochondrial and a cytoplasmic isoform.
UniProt Protein Details:

Protein type:Other Amino Acids Metabolism - glutathione; Oxidoreductase; EC 1.8.1.7

Chromosomal Location of Human Ortholog: 8p21.1

Cellular Component: cytosol; mitochondrial matrix

Molecular Function:electron carrier activity; glutathione-disulfide reductase activity

Biological Process: nucleobase, nucleoside and nucleotide interconversion; response to reactive oxygen species

NCBI Summary:This gene encodes a member of the class-I pyridine nucleotide-disulfide oxidoreductase family. This enzyme is a homodimeric flavoprotein. It is a central enzyme of cellular antioxidant defense, and reduces oxidized glutathione disulfide (GSSG) to the sulfhydryl form GSH, which is an important cellular antioxidant. Rare mutations in this gene result in hereditary glutathione reductase deficiency. Multiple alternatively spliced transcript variants encoding different isoforms have been found. [provided by RefSeq, Aug 2010]
UniProt Code:P00390
NCBI GenInfo Identifier:14916998
NCBI Gene ID:2936
NCBI Accession:P00390.2
UniProt Secondary Accession:P00390,Q7Z5C9, Q9NP63, C8KIL8, C8KIL9, C8KIM0, D3DSV3
UniProt Related Accession:P00390
Molecular Weight:47,267 Da
NCBI Full Name:Glutathione reductase, mitochondrial
NCBI Synonym Full Names:glutathione-disulfide reductase
NCBI Official Symbol:GSR��
NCBI Official Synonym Symbols:HEL-75; HEL-S-122m��
NCBI Protein Information:glutathione reductase, mitochondrial
UniProt Protein Name:Glutathione reductase, mitochondrial
Protein Family:Glutathione reductase
UniProt Gene Name:GSR��
UniProt Entry Name:GSHR_HUMAN

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