Human GSTM3 Recombinant Protein (RPPB1766)
- SKU:
- RPPB1766
- Product Type:
- Recombinant Protein
- Species:
- Human
- Uniprot:
- P21266
- Research Area:
- Enzymes
Description
| Product Name: | Human GSTM3 Recombinant Protein |
| Product Code: | RPPB1766 |
| Size: | 20µg |
| Species: | Human |
| Target: | GSTM3 |
| Synonyms: | Glutathione S-transferase mu 3 (brain), GST5, GSTM3-3, GST class-mu 3, GSTB, GTM3, brain type mu-glutathione S-transferase, glutathione S-aralkyltransferase M3, glutathione S-alkyltransferase M3, S-(hydroxyalkyl)glutathione lyase M3, EC 2.5.1.18. |
| Source: | Escherichia Coli |
| Physical Appearance: | Sterile Filtered colorless solution. |
| Formulation: | The GSTM3 solution (1mg/ml) contains 20mM Tris-HCl buffer (pH 8.0), 100mM Nacl,1mM DTT and 10% glycerol. |
| Stability: | Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles. |
| Purity: | Greater than 95% as determined by SDS-PAGE. |
| Amino Acid Sequence: | MGSSHHHHHH SSGLVPRGSH MGSHMSCESS MVLGYWDIRG LAHAIRLLLE FTDTSYEEKR YTCGEAPDYD RSQWLDVKFK LDLDFPNLPY LLDGKNKITQ SNAILRYIAR KHNMCGETEE EKIRVDIIEN QVMDFRTQLI RLCYSSDHEK LKPQYLEELP GQLKQFSMFL GKFSWFAGEK LTFVDFLTYD ILDQNRIFDP KCLDEFPNLK AFMCRFEALE KIAAYLQSDQ FCKMPINNKM AQWGNKPVC |
GSTM3 belongs to the glutathione s-transferase (GST) family of proteins. There are eight GST proteins families, named alpha, kappa, mu, omega, pi, sigma, theta and zeta. Each one of the GST families has several proteins with different functions throughout the cell. The mu type of enzymes takes part in the detoxification of electrophilic compounds, including therapeutic drugs, carcinogens, environmental toxins and products of oxidative stress, by conjugation with glutathione.
GSTM3 Recombinant produced in E. coli is a single polypeptide chain containing 249 amino acids (1-225) and having a molecular mass of 29.1kDa.GSTM3 is fused to a 24 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
| UniProt Protein Function: | GSTM3: Conjugation of reduced glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles. May govern uptake and detoxification of both endogenous compounds and xenobiotics at the testis and brain blood barriers. Belongs to the GST superfamily. Mu family. |
| UniProt Protein Details: | Protein type:Transferase; Other Amino Acids Metabolism - glutathione; Xenobiotic Metabolism - drug metabolism - cytochrome P450; Cell development/differentiation; Xenobiotic Metabolism - metabolism by cytochrome P450; EC 2.5.1.18 Chromosomal Location of Human Ortholog: 1p13.3 Cellular Component: cytoplasm; cytosol; nucleus Molecular Function:identical protein binding; protein homodimerization activity; enzyme binding; glutathione binding; glutathione transferase activity Biological Process: glutathione metabolic process; establishment of blood-nerve barrier; nitrobenzene metabolic process; response to estrogen stimulus; xenobiotic metabolic process; xenobiotic catabolic process |
| NCBI Summary: | Cytosolic and membrane-bound forms of glutathione S-transferase are encoded by two distinct supergene families. At present, eight distinct classes of the soluble cytoplasmic mammalian glutathione S-transferases have been identified: alpha, kappa, mu, omega, pi, sigma, theta and zeta. This gene encodes a glutathione S-transferase that belongs to the mu class. The mu class of enzymes functions in the detoxification of electrophilic compounds, including carcinogens, therapeutic drugs, environmental toxins and products of oxidative stress, by conjugation with glutathione. The genes encoding the mu class of enzymes are organized in a gene cluster on chromosome 1p13.3 and are known to be highly polymorphic. These genetic variations can change an individual's susceptibility to carcinogens and toxins as well as affect the toxicity and efficacy of certain drugs. Mutations of this class mu gene have been linked with a slight increase in a number of cancers, likely due to exposure with environmental toxins. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Nov 2008] |
| UniProt Code: | P21266 |
| NCBI GenInfo Identifier: | 21264423 |
| NCBI Gene ID: | 2947 |
| NCBI Accession: | P21266.3 |
| UniProt Related Accession: | P21266 |
| Molecular Weight: | 27kDa |
| NCBI Full Name: | Glutathione S-transferase Mu 3 |
| NCBI Synonym Full Names: | glutathione S-transferase mu 3 |
| NCBI Official Symbol: | GSTM3�� |
| NCBI Official Synonym Symbols: | GST5; GSTB; GTM3; GSTM3-3�� |
| NCBI Protein Information: | glutathione S-transferase Mu 3 |
| UniProt Protein Name: | Glutathione S-transferase Mu 3 |
| UniProt Synonym Protein Names: | GST class-mu 3; GSTM3-3; hGSTM3-3 |
| Protein Family: | Glutathione S-transferase |
| UniProt Gene Name: | GSTM3�� |
| UniProt Entry Name: | GSTM3_HUMAN |
