Human GSTZ1 Recombinant Protein (RPPB1779)
- SKU:
- RPPB1779
- Product Type:
- Recombinant Protein
- Species:
- Human
- Uniprot:
- O43708
- Research Area:
- Enzymes
Description
| Product Name: | Human GSTZ1 Recombinant Protein |
| Product Code: | RPPB1779 |
| Size: | 25µg |
| Species: | Human |
| Target: | GSTZ1 |
| Synonyms: | MAAI, GSTZ-1, MAI, Maleylacetone Isomerase, EC 2.5.1.18, Maleylacetoacetate isomerase, Glutathione S-transferase zeta 1, EC 5.2.1.2, GSTZ1-1, MGC2029, GSTZ1. |
| Source: | Escherichia Coli |
| Physical Appearance: | Sterile Filtered colorless solution. |
| Formulation: | The GSTZ1 protein solution contains 1x PBS pH-7.4 and 10% glycerol. |
| Stability: | Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles. |
| Purity: | Greater than 95.0% as determined by SDS-PAGE. |
| Amino Acid Sequence: | MGSSHHHHHH SSGLVPRGSH MQAGKPILYS YFRSSCSWRV RIALALKGID YETVPINLIK DGGQQFSKDF QALNPMKQVP TLKIDGITIH QSLAIIEYLE ETRPTPRLLP QDPKKRASVR MISDLIAGGI QPLQNLSVLK QVGEEMQLTW AQNAITCGFN ALEQILQSTA GIYCVGDEVT MADLCLVPQV ANAERFKVDL TPYPTISSIN KRLLVLEAFQ VSHPCRQPDT PTELRA |
GSTZ1 is part of the glutathione S-transferase super-family which encodes multifunctional enzymes vital in the detoxification of electrophilic molecules, including carcinogens, mutagens, and several therapeutic drugs, by conjugation with glutathione. GSTZ1 participates in the catabolism of phenylalanine and tyrosine. Thus defects in GSTZ1 cause harsh metabolic disorders including alkaptonuria, phenylketonuria and tyrosinaemia. GSTZ1 is a bifunctional protein which has minimal glutathione-conjugating activity with 7-chloro-4-nitrobenz-2-oxa-1,3-diazole and maleylacetoacetate isomerase activity. GSTZ1 has low glutathione peroxidase activity with T-butyl and cumene hydroperoxides. GSTZ1 catalyzes the glutathione dependent oxygenation of dichloroacetic acid to glyoxylic acid.
GSTZ1 Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 236 amino acids (1-216 a.a.) and having a molecular mass of 26.2 kDa. The GSTZ1 is fused to a 20 amino acid His-Tag at N-terminus and purified by conventional chromatography.
| UniProt Protein Function: | GSTZ1: Bifunctional enzyme showing minimal glutathione- conjugating activity with ethacrynic acid and 7-chloro-4- nitrobenz-2-oxa-1,3-diazole and maleylacetoacetate isomerase activity. Has also low glutathione peroxidase activity with T- butyl and cumene hydroperoxides. Is able to catalyze the glutathione dependent oxygenation of dichloroacetic acid to glyoxylic acid. Belongs to the GST superfamily. Zeta family. 2 isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Protein type:Xenobiotic Metabolism - metabolism by cytochrome P450; EC 5.2.1.2; Isomerase; Transferase; EC 2.5.1.18; Xenobiotic Metabolism - drug metabolism - cytochrome P450; Amino Acid Metabolism - tyrosine; Mitochondrial; Oxidoreductase; Other Amino Acids Metabolism - glutathione Chromosomal Location of Human Ortholog: 14q24.3 Cellular Component: mitochondrion; cytosol Molecular Function:protein binding; protein homodimerization activity; glutathione transferase activity; maleylacetoacetate isomerase activity; glutathione peroxidase activity Biological Process: L-phenylalanine catabolic process; glutathione metabolic process; tyrosine catabolic process; xenobiotic metabolic process |
| NCBI Summary: | This gene is a member of the glutathione S-transferase (GSTs) super-family which encodes multifunctional enzymes important in the detoxification of electrophilic molecules, including carcinogens, mutagens, and several therapeutic drugs, by conjugation with glutathione. This enzyme also plays a significant role in the catabolism of phenylalanine and tyrosine. Thus defects in this enzyme may lead to severe metabolic disorders including alkaptonuria, phenylketonuria and tyrosinaemia. Several transcript variants of this gene encode multiple protein isoforms. [provided by RefSeq, Jul 2008] |
| UniProt Code: | O43708 |
| NCBI GenInfo Identifier: | 269849671 |
| NCBI Gene ID: | 2954 |
| NCBI Accession: | O43708.3 |
| UniProt Secondary Accession: | O43708,O15308, O75430, Q6IB17, Q7Z610, Q9BV63, A6NED0 A6NNB8, A8MWD7, B2RCK3, |
| UniProt Related Accession: | O43708 |
| Molecular Weight: | 216 |
| NCBI Full Name: | Maleylacetoacetate isomerase |
| NCBI Synonym Full Names: | glutathione S-transferase zeta 1 |
| NCBI Official Symbol: | GSTZ1�� |
| NCBI Official Synonym Symbols: | MAI; MAAI; GSTZ1-1�� |
| NCBI Protein Information: | maleylacetoacetate isomerase; maleylacetone isomerase; glutathione S-aryltransferase; glutathione S-alkyltransferase; glutathione transferase zeta 1; glutathione S-aralkyltransferase; S-(hydroxyalkyl)glutathione lyase |
| UniProt Protein Name: | Maleylacetoacetate isomerase |
| UniProt Synonym Protein Names: | GSTZ1-1; Glutathione S-transferase zeta 1 (EC:2.5.1.18) |
| Protein Family: | Glutathione S-transferase |
| UniProt Gene Name: | GSTZ1�� |
| UniProt Entry Name: | MAAI_HUMAN |
