Human HAT1 Recombinant Protein (RPPB1799)
- SKU:
- RPPB1799
- Product Type:
- Recombinant Protein
- Species:
- Human
- Uniprot:
- O14929
- Research Area:
- Enzymes
Description
| Product Name: | Human HAT1 Recombinant Protein |
| Product Code: | RPPB1799 |
| Size: | 10µg |
| Species: | Human |
| Target: | HAT1 |
| Synonyms: | EC 2.3.1.48, KAT1, histone acety ltransferase 1, Histone acetyltransferase type B catalytic subunit, HAT1. |
| Source: | Escherichia Coli |
| Physical Appearance: | Sterile Filtered clear colorless solution. |
| Formulation: | The HAT1 solution contains 20mM Tris-HCl pH-8, 1mM DTT, and 10% glycerol. |
| Stability: | Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles. |
| Purity: | Greater than 90% as determined by SDS-PAGE. |
| Amino Acid Sequence: | MGSSHHHHHH SSGLVPRGSH MKKLAEYKCN TNTAIELKLV RFPEDLENDI RTFFPEYTHQ LFGDDETAFG YKGLKILLYY IAGSLSTMFR VEYASKVDEN FDCVEADDVE GKIRQIIPPG FCTNTNDFLS LLEKEVDFKP FGTLLHTYSV LSPTGGENFT FQIYKADMTC RGFREYHERL QTFLMWFIET ASFIDVDDER WHYFLVFEKY NKDGATLFAT VGYMTVYNYY VYPDKTRPRV SQMLILTPFQ GQGHGAQLLE TVHRYYTEFP TVLDITAEDP SKSYVKLRDF VLVKLCQDLP CFSREKLMQG FNEDMAIEAQ QKFKINKQHA RRVYEILRLL VTD |
HAT1 is a type B histone acetyltransferase that participates in the rapid acetylation of newly synthesized cytoplasmic histones, which are in turn imported into the nucleus for de novo deposition onto nascent DNA chains. Histone acetylation, mainly of soluble histone H4 at ''Lys-5'' and ''Lys-12'' and acetylates histone H2A at ''Lys-5''. HAT1 takes part in replication-dependent chromatin assembly. HAT1 acetylates soluble but not nucleosomal histone H4 at lysines 5 and 12, and to a lesser degree, histone H2A at lysine 5. HAT1 has intrinsic substrate specificity that modifies lysine in recognition sequence GXGKXG.
HAT1 Human Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 343 amino acids (20-341 a.a.) and having a molecular mass of 40.1 kDa. The HAT1 is fused to a 21 amino acid His Tag and purified by proprietary chromatographic techniques.
| UniProt Protein Function: | HAT1: Acetylates soluble but not nucleosomal histone H4 at 'Lys-5' (H4K5ac) and 'Lys-12' (H4K12ac) and, to a lesser extent, acetylates histone H2A at 'Lys-5' (H2AK5ac). Has intrinsic substrate specificity that modifies lysine in recognition sequence GXGKXG. May be involved in nucleosome assembly during DNA replication and repair as part of the histone H3.1 and H3.3 complexes. May play a role in DNA repair in response to free radical damage. Belongs to the HAT1 family. 2 isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Protein type:Acetyltransferase; EC 2.3.1.48 Chromosomal Location of Human Ortholog: 2q31.2-q33.1 Cellular Component: intracellular membrane-bound organelle; nuclear chromatin; nuclear chromosome, telomeric region; nucleoplasm; nucleus; protein complex Molecular Function:H4 histone acetyltransferase activity; histone acetyltransferase activity; histone binding; protein binding Biological Process: DNA packaging; DNA replication-dependent nucleosome assembly; DNA replication-independent nucleosome assembly; internal protein amino acid acetylation |
| NCBI Summary: | The protein encoded by this gene is a type B histone acetyltransferase (HAT) that is involved in the rapid acetylation of newly synthesized cytoplasmic histones, which are in turn imported into the nucleus for de novo deposition onto nascent DNA chains. Histone acetylation, particularly of histone H4, plays an important role in replication-dependent chromatin assembly. Specifically, this HAT can acetylate soluble but not nucleosomal histone H4 at lysines 5 and 12, and to a lesser degree, histone H2A at lysine 5. Alternatively spliced transcript variants have been identified for this gene. [provided by RefSeq, Jun 2009] |
| UniProt Code: | O14929 |
| NCBI GenInfo Identifier: | 3334209 |
| NCBI Gene ID: | 8520 |
| NCBI Accession: | O14929.1 |
| UniProt Secondary Accession: | O14929,Q49A44, Q53QF0, Q53SU4, Q6P594, Q8WWB9, |
| UniProt Related Accession: | O14929 |
| Molecular Weight: | 39,786 Da |
| NCBI Full Name: | Histone acetyltransferase type B catalytic subunit |
| NCBI Synonym Full Names: | histone acetyltransferase 1 |
| NCBI Official Symbol: | HAT1�� |
| NCBI Official Synonym Symbols: | KAT1�� |
| NCBI Protein Information: | histone acetyltransferase type B catalytic subunit |
| UniProt Protein Name: | Histone acetyltransferase type B catalytic subunit |
| UniProt Synonym Protein Names: | Histone acetyltransferase 1 |
| Protein Family: | HAT1-interacting factor |
| UniProt Gene Name: | HAT1�� |
| UniProt Entry Name: | HAT1_HUMAN |
