The Human Hepc25 (Hepcidin-25) ELISA Kit is specifically designed for the precise measurement of hepcidin-25 levels in human serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, ensuring consistent and dependable results for a variety of research purposes.Hepcidin-25 is a key regulator of iron metabolism, playing a crucial role in controlling iron absorption and distribution in the body. Dysregulation of hepcidin-25 levels has been linked to various disorders such as anemia, hemochromatosis, and inflammatory conditions, making it a vital biomarker for studying these diseases and developing potential treatments. With the Human Hepc25 ELISA Kit, researchers can accurately assess hepcidin-25 levels in biological samples, helping to deepen our understanding of iron homeostasis and its impact on various health conditions. This kit provides a valuable tool for advancing research in the field of iron metabolism and related diseases.
Product Name:
Human Hepc25 (Hepcidin 25) ELISA Kit
SKU:
HUES03477
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Sandwich
Assay type:
Sandwich-ELISA
Assay time:
3 h 30 min
Sensitivity:
0.94 ng/mL
Detection range:
1.56-100 ng/mL
Reovery:
80%-120%
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to the target protein. Standards or samples are added to the micro ELISA plate wells and bind to the immobilized antibody. A biotinylated detection antibody specific to the target protein is then added, followed by Avidin-Horseradish Peroxidase (HRP) conjugate. Free components are washed away. The substrate solution is added to each well, resulting in a color change. Only wells containing the target protein, detection antibody, and HRP conjugate will develop a blue color. The reaction is terminated by the addition of stop solution, resulting in a yellow color. The optical density (OD) is measured at 450 nm ± 2 nm. The OD value is directly proportional to the concentration of the target protein in the sample and is determined using a standard curve.
