Human HIF-1alpha (Hypoxia Inducible Factor 1 Alpha) ELISA Kit
The Human HIF-1α (Hypoxia Inducible Factor 1 Alpha) ELISA Kit is a specialized assay designed for the quantitative detection of HIF-1α levels in various human biological samples. HIF-1α is a key transcription factor that plays a crucial role in the cellular response to hypoxia, regulating the expression of genes involved in critical processes such as angiogenesis, metabolism, and cell survival.
Accurate measurement of HIF-1α is essential for studying oxygen homeostasis, cellular adaptation to low oxygen conditions, and the pathophysiology of multiple diseases. Our HIF-1α ELISA Kit offers exceptional sensitivity and specificity, ensuring precise and reproducible results. With stringent quality control measures in place, this kit provides reliable performance and user-friendly protocols, making it an excellent choice for both research and clinical applications. Trust Assay Genie's HIF-1α ELISA Kit for accurate and dependable quantification of this important biomarker in your studies.
Product Name:
Human HIF-1alpha (Hypoxia Inducible Factor 1 Alpha) ELISA Kit
SKU:
AEES00102
Target:
Human HIF-1α (Hypoxia Inducible Factor 1 Alpha)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
37.50 pg/mL
Detection range:
62.50-4000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human HIF-1α. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human HIF-1α and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human HIF-1α, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human HIF-1α. You can calculate the concentration of Human HIF-1α in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
88-101
94-106
91-102
Average (%)
94
101
98
1:4
Range (%)
93-107
94-104
92-105
Average (%)
99
100
100
1:8
Range (%)
86-98
90-100
96-110
Average (%)
92
94
102
1:16
Range (%)
94-108
99-107
84-99
Average (%)
101
102
91
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
94-109
102
EDTA plasma (n=5)
99-109
104
Cell culture media (n=5)
101-112
105
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
177.29
422.96
1738.08
194.33
486.58
1980.59
Standard deviation
9.41
20.26
71.09
10.47
24.09
91.11
C V (%)
5.31
4.79
4.09
5.39
4.95
4.6
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human HIF-1α concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human HIF-1α in samples. No significant cross-reactivity or interference between Human HIF-1α and analogues was observed.
