Human HMGB-1 (High mobility group protein B1) CLIA Kit
The Human HMGB-1 (High Mobility Group Protein B1) CLIA Kit is designed for the precise detection of HMGB-1 levels in human samples such as serum, plasma, and cell culture supernatants. This highly sensitive and specific kit ensures accurate and consistent results, making it an excellent tool for a variety of research applications.HMGB-1 is a critical protein involved in inflammation, immune response, and tissue repair.
Elevated levels of HMGB-1 have been associated with various diseases including sepsis, arthritis, and cancer, making it a valuable biomarker for studying these conditions and developing potential treatments.With its reliable performance and broad range of applications, the Human HMGB-1 CLIA Kit from Assay Genie is an essential tool for researchers looking to investigate the role of HMGB-1 in health and disease.
Product Name:
Human HMGB-1 (High mobility group protein B1) CLIA Kit
SKU:
HUES00881
Target:
Human HMGB-1 (High mobility group protein B1)
Size:
96T
Assay type:
Sandwich-CLIA
Assay time:
3.5h
Sensitivity:
7.50 pg/mL
Detection range:
12.50-800 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human HMGB-1. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human HMGB-1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human HMGB-1, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human HMGB-1. You can calculate the concentration of Human HMGB-1 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
93-109
103-118
103-120
Average (%)
101
109
109
1:4
Range (%)
95-111
94-107
85-98
Average (%)
103
99
90
1:8
Range (%)
93-109
86-100
101-114
Average (%)
99
93
108
1:16
Range (%)
88-101
85-99
88-101
Average (%)
95
91
94
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
99-111
104
EDTA plasma (n=5)
94-111
102
Cell culture media (n=5)
86-99
91
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
38.94
71.2
397.79
37.0
72.83
379.26
Standard deviation
4.74
8.16
27.92
3.48
5.48
28.71
C V (%)
12.17
11.46
7.02
9.41
7.52
7.57
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human HMGB-1 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human HMGB-1 in samples. No significant cross-reactivity or interference between Human HMGB-1 and analogues was observed.
