Human HMGB-1 (High mobility group protein B1) ELISA Kit
The Human HMGB-1 (High Mobility Group Protein B1) ELISA Kit is a highly specific and sensitive assay designed for the accurate measurement of HMGB-1 levels in human serum, plasma, and cell culture supernatants. This kit offers reliable and reproducible results, making it a valuable tool for a variety of research applications.HMGB-1 is a critical protein involved in various inflammatory and immune responses, playing a key role in conditions such as sepsis, arthritis, and cancer.
By accurately measuring HMGB-1 levels, researchers can gain insights into disease progression, treatment efficacy, and potential therapeutic strategies.With its high sensitivity and specificity, the Human HMGB-1 ELISA Kit is an essential tool for studying the role of HMGB-1 in health and disease, offering researchers a reliable and efficient method for quantifying HMGB-1 levels in biological samples.
Product Name:
Human HMGB-1 (High mobility group protein B1) ELISA Kit
SKU:
HUES02568
Target:
Human HMGB-1 (High mobility group protein B1)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human HMGB-1. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human HMGB-1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human HMGB-1, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human HMGB-1. You can calculate the concentration of Human HMGB-1 in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
98-111
92-105
85-100
Average (%)
105
98
92
1:4
Range (%)
90-103
84-100
88-102
Average (%)
98
91
94
1:8
Range (%)
87-99
83-93
85-96
Average (%)
94
88
90
1:16
Range (%)
88-103
85-96
86-99
Average (%)
94
90
91
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
94-108
100
EDTA plasma (n=5)
94-107
100
Cell culture media (n=5)
93-105
99
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
99.32
202.75
693.26
108.96
208.03
746.59
Standard deviation
5.84
8.62
35.56
5.86
9.76
26.06
C V (%)
5.88
4.25
5.13
5.38
4.69
3.49
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human HMGB-1 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human HMGB-1 in samples. No significant cross-reactivity or interference between Human HMGB-1 and analogues was observed.
