Human HSP-27/HSPB1 (Heat Shock Protein 27) CLIA Kit
The Human HSP-27 (HSPB1) CLIA Kit is specifically designed for the accurate detection of heat shock protein 27 levels in human samples such as serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, ensuring precise and consistent results for a variety of research applications.Heat shock protein 27, also known as HSPB1, is a key player in cellular stress response and is involved in various biological processes such as cell survival, apoptosis, and protein folding. Dysregulation of HSP-27 has been linked to a range of diseases including cancer, neurodegenerative disorders, and cardiovascular diseases, highlighting its importance as a potential biomarker for disease progression and therapeutic development.
With the Human HSP-27 CLIA Kit, researchers can accurately measure HSP-27 levels in human samples, allowing for in-depth investigations into its role in disease pathogenesis and potential as a therapeutic target. The reliable performance and robust design of this kit make it an essential tool for studying the intricacies of heat shock protein 27 biology.
Product Name:
Human HSP-27/HSPB1 (Heat Shock Protein 27) CLIA Kit
SKU:
HUES01027
Target:
Human HSP-27/HSPB1 (Heat Shock Protein 27)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
0.09 ng/mL
Detection range:
0.16-10 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human HSP-27/HSPB1. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human HSP-27/HSPB1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human HSP-27/HSPB1, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human HSP-27/HSPB1. You can calculate the concentration of Human HSP-27/HSPB1 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
99-113
88-103
99-116
Average (%)
104
95
106
1:4
Range (%)
100-115
91-103
95-109
Average (%)
108
97
102
1:8
Range (%)
95-108
100-119
101-118
Average (%)
101
109
107
1:16
Range (%)
96-109
86-97
102-115
Average (%)
102
92
108
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
89-106
97
EDTA plasma (n=5)
93-109
101
Cell culture media (n=5)
85-97
92
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
0.5
0.86
4.41
0.51
0.87
4.51
Standard deviation
0.06
0.08
0.5
0.06
0.07
0.35
C V (%)
12.0
9.3
11.34
11.76
8.05
7.76
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human HSP-27/HSPB1 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human HSP-27/HSPB1 in samples. No significant cross-reactivity or interference between Human HSP-27/HSPB1 and analogues was observed.
