The Human HSP-70 (Heat Shock Protein 70) ELISA Kit is specifically designed for the precise measurement of HSP-70 levels in human samples including serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, ensuring accurate and consistent results for a variety of research applications.HSP-70 is a key protein involved in cellular stress response, playing a vital role in protecting cells from damage and promoting cell survival.
Dysregulation of HSP-70 has been implicated in various diseases, including cancer, neurodegenerative disorders, and cardiovascular diseases, making it a valuable biomarker for studying these conditions and potential therapeutic interventions.Overall, the Human HSP-70 ELISA Kit provides researchers with a reliable tool to investigate the role of HSP-70 in disease pathogenesis and therapeutic development.
Product Name:
Human HSP-70 (Heat Shock Protein 70) ELISA Kit
SKU:
HUES02815
Target:
Human HSP-70 (Heat Shock Protein 70)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
0.47 ng/mL
Detection range:
0.78-50 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human HSP-70/HSPA9. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human HSP-70/HSPA9 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human HSP-70/HSPA9, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human HSP-70/HSPA9. You can calculate the concentration of Human HSP-70/HSPA9 in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
84-96
89-101
89-106
Average (%)
91
94
96
1:4
Range (%)
103-119
80-92
90-104
Average (%)
108
86
98
1:8
Range (%)
93-107
85-95
92-108
Average (%)
100
90
99
1:16
Range (%)
100-115
83-93
93-106
Average (%)
108
87
101
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
93-108
100
EDTA plasma (n=5)
91-106
97
Cell culture media (n=5)
91-103
98
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
2.3
6.0
22.6
2.3
5.6
24.1
Standard deviation
0.1
0.3
1.1
0.1
0.3
0.9
C V (%)
4.35
5.0
4.87
4.35
5.36
3.73
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human HSP-70/HSPA9 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human HSP-70/HSPA9 in samples. No significant cross-reactivity or interference between Human HSP-70/HSPA9 and analogues was observed.
