The Human Hsp-90 (Heat Shock Protein 90) ELISA Kit is designed to accurately measure levels of Hsp-90 in human serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, ensuring dependable and consistent results for a variety of research purposes.Hsp-90 is a key molecular chaperone that plays a critical role in protein folding, stability, and function. It is involved in various cellular processes, including cell cycle regulation, signal transduction, and stress responses. Dysregulation of Hsp-90 has been associated with cancer, neurodegenerative diseases, and other disorders, making it a valuable biomarker for investigating these conditions and exploring potential therapeutic interventions. With the Human Hsp-90 ELISA Kit, researchers can accurately quantify Hsp-90 levels in biological samples, allowing for better understanding of its role in health and disease. This kit is easy to use, reliable, and delivers precise results, making it an essential tool for biomedical research and drug development efforts.
Product Name:
Human HSP-90 (Heat Shock Protein 90) ELISA Kit
SKU:
HUES02816
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Sandwich
Assay type:
Sandwich-ELISA
Assay time:
3 h 30 min
Sensitivity:
1.88 ng/mL
Detection range:
3.13-200 ng/mL
Reovery:
80%-120%
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to the target protein. Standards or samples are added to the micro ELISA plate wells and bind to the immobilized antibody. A biotinylated detection antibody specific to the target protein is then added, followed by Avidin-Horseradish Peroxidase (HRP) conjugate. Free components are washed away. The substrate solution is added to each well, resulting in a color change. Only wells containing the target protein, detection antibody, and HRP conjugate will develop a blue color. The reaction is terminated by the addition of stop solution, resulting in a yellow color. The optical density (OD) is measured at 450 nm ± 2 nm. The OD value is directly proportional to the concentration of the target protein in the sample and is determined using a standard curve.
