The Human IDE (Insulin-Degrading Enzyme) CLIA Kit is designed for the precise measurement of IDE levels in human serum, plasma, and cell culture samples. This kit offers exceptional sensitivity and specificity, ensuring accurate and consistent results for a variety of research purposes.IDE is a key enzyme involved in the breakdown of insulin and amyloid beta, making it a crucial player in maintaining glucose homeostasis and potentially impacting neurodegenerative diseases such as Alzheimer's. By accurately measuring IDE levels, researchers can better understand its role in disease pathology and potentially identify new therapeutic targets.Overall, the Human IDE CLIA Kit is a valuable tool for studying the role of IDE in various diseases and exploring potential treatment options. Its reliable performance and ease of use make it a valuable asset for any laboratory conducting research in the field of insulin degradation and related disorders.
Product Name:
Human IDE (Insulin Degrading Enzyme) CLIA Kit
SKU:
HUES00310
Size:
96 Assays
Detection Method:
Chemiluminescence
Assay type:
Sandwich-CLIA
Assay time:
3 h 30 min
Sensitivity:
9.38 pg/mL
Detection range:
15.63-1000 pg/mL
Reovery:
80%-120%
This kit uses a sandwich chemiluminescence immunoassay (CLIA) principle. The microplate is pre-coated with an antibody specific to the target protein. Standards or samples are added to the wells and bind to the immobilized antibody. A biotinylated detection antibody is then added, followed by HRP-conjugated streptavidin to form a sandwich complex. After washing to remove unbound components, a chemiluminescent substrate is added. The HRP enzyme catalyzes a light-emitting reaction. The intensity of the emitted light is directly proportional to the concentration of the target protein in the sample. The signal is measured using a luminometer, and the concentration of the analyte is calculated based on a standard curve.
