The Human IL-6 ELISA Kit is meticulously developed for the quantitative assessment of Interleukin-6 (IL-6) levels in a diverse range of biological samples. IL-6 is a vital pro-inflammatory cytokine involved in numerous cellular functions, including immune modulation, hematopoiesis, and acute-phase responses. Its role in mediating inflammatory processes and immunity makes accurate IL-6 measurement crucial for understanding immune regulation, inflammatory diseases, and the development of effective therapeutic strategies. Our IL-6 ELISA Kit boasts exceptional sensitivity and specificity, ensuring precise and reproducible results for your research needs. With stringent quality control measures, this kit guarantees robust performance and ease of use, making it an optimal choice for high-quality research applications. Trust Assay Genie's IL-6 ELISA Kit for accurate and dependable quantification of this pivotal cytokine in your studies.
Product Name:
Human IL-6 ELISA Kit
SKU:
AEES00163
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Sandwich
Assay type:
Sandwich-ELISA
Assay time:
3 h 30 min
Sensitivity:
0.94 pg/mL
Detection range:
1.56-100 pg/mL
Reovery:
80%-120%
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to the target protein. Standards or samples are added to the micro ELISA plate wells and bind to the immobilized antibody. A biotinylated detection antibody specific to the target protein is then added, followed by Avidin-Horseradish Peroxidase (HRP) conjugate. Free components are washed away. The substrate solution is added to each well, resulting in a color change. Only wells containing the target protein, detection antibody, and HRP conjugate will develop a blue color. The reaction is terminated by the addition of stop solution, resulting in a yellow color. The optical density (OD) is measured at 450 nm ± 2 nm. The OD value is directly proportional to the concentration of the target protein in the sample and is determined using a standard curve.
