The Human LEPR (Leptin Receptor) ELISA Kit by Assay Genie is expertly crafted for the precise quantitative measurement of Leptin Receptor (LEPR) levels in a variety of biological samples. LEPR, as a key player in the regulation of energy balance and metabolism, is integrally involved in modulation of appetite, energy expenditure, and adipogenesis. Understanding the intricacies of LEPR signaling pathways is crucial in unraveling the mechanisms underpinning metabolic disorders and obesity-related complications. Our LEPR ELISA Kit stands out for its exceptional sensitivity and specificity, guaranteeing accurate and reproducible results in your research endeavors. With stringent quality control measures in place, this kit assures robust performance, making it an ideal choice for conducting cutting-edge research studies. Trust Assay Genie's LEPR ELISA Kit for reliable and insightful data insights that drive scientific discovery.
Product Name:
Human LEPR (Leptin Receptor) ELISA Kit
SKU:
AEES00174
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Sandwich
Assay type:
Sandwich-ELISA
Assay time:
3 h 30 min
Sensitivity:
0.38 ng/mL
Detection range:
0.63-40 ng/mL
Reovery:
80%-120%
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to the target protein. Standards or samples are added to the micro ELISA plate wells and bind to the immobilized antibody. A biotinylated detection antibody specific to the target protein is then added, followed by Avidin-Horseradish Peroxidase (HRP) conjugate. Free components are washed away. The substrate solution is added to each well, resulting in a color change. Only wells containing the target protein, detection antibody, and HRP conjugate will develop a blue color. The reaction is terminated by the addition of stop solution, resulting in a yellow color. The optical density (OD) is measured at 450 nm ± 2 nm. The OD value is directly proportional to the concentration of the target protein in the sample and is determined using a standard curve.
