Description
| Product Name: | Human MAFG Recombinant Protein |
| Product Code: | RPPB3908 |
| Size: | 10µg |
| Species: | Human |
| Target: | MAFG |
| Synonyms: | Transcription factor MafG, V-maf musculoaponeurotic fibrosarcoma oncogene homolog G, hMAF, MAFG, hMAF. |
| Source: | Escherichia Coli |
| Physical Appearance: | Sterile filtered colorless solution. |
| Formulation: | The MAFG solution (0.25mg/ml) contains 20mM Tris-HCl buffer (pH8.0), 40% glycerol, 0.15M NaCl and 1mM DTT. |
| Stability: | Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles. |
| Purity: | Greater than 90% as determined by SDS-PAGE. |
| Amino Acid Sequence: | MGSSHHHHHH SSGLVPRGSH MTTPNKGNKA LKVKREPGEN GTSLTDEELV TMSVRELNQH LRGLSKEEIV QLKQRRRTLK NRGYAASCRV KRVTQKEELE KQKAELQQEV EKLASENASM KLELDALRSK YEALQTFART VARSPVAPAR GPLAAGLGPL VPGKVAATSV ITIVKSKTDA RS |
Transcription factor MafG (MAFG) is a member of the Maf family. Given that they lack a putative transactivation domain, the small Mafs act as transcriptional repressors when they dimerize amongst themselves. Nevertheless, they seem to function as transcriptional activators by dimerizing with other (generally larger) basic-zipper proteins and engaging them to specific DNA-binding sites. Small Maf proteins heterodimerize with Fos and may function as competitive repressors of the NF-E2 transcription factor.
MAFG Human Recombinant produced in E.coli is a single, non-glycosylated polypeptide chain containing 182 amino acids (1-162) and having a molecular mass of 20kDa.MAFG is fused to a 20 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
| UniProt Protein Function: | MAFG: Since they lack a putative transactivation domain, the small Mafs behave as transcriptional repressors when they dimerize among themselves. However, they seem to serve as transcriptional activators by dimerizing with other (usually larger) basic-zipper proteins and recruiting them to specific DNA-binding sites. Small Maf proteins heterodimerize with Fos and may act as competitive repressors of the NF-E2 transcription factor. Transcription factor, component of erythroid-specific transcription factor NF- E2. Activates globin gene expression when associated with NF-E2. May be involved in signal transduction of extracellular H(+). Belongs to the bZIP family. Maf subfamily. |
| UniProt Protein Details: | Protein type:DNA-binding Chromosomal Location of Human Ortholog: 17q25.3 Cellular Component: nucleoplasm; nucleus Molecular Function:protein binding Biological Process: blood coagulation; regulation of transcription from RNA polymerase II promoter |
| NCBI Summary: | Globin gene expression is regulated through nuclear factor erythroid-2 (NFE2) elements located in enhancer-like locus control regions positioned many kb upstream of alpha- and beta-gene clusters (summarized by Blank et al., 1997 [PubMed 9166829]). NFE2 DNA-binding activity consists of a heterodimer containing a ubiquitous small Maf protein (MafF, MIM 604877; MafG; or MafK, MIM 600197) and the tissue-restricted protein p45 NFE2 (MIM 601490). Both subunits are members of the activator protein-1-like superfamily of basic leucine zipper (bZIP) proteins (see MIM 165160).[supplied by OMIM, Mar 2010] |
| UniProt Code: | O15525 |
| NCBI GenInfo Identifier: | 6685623 |
| NCBI Gene ID: | 4097 |
| NCBI Accession: | O15525.1 |
| UniProt Related Accession: | O15525 |
| Molecular Weight: | 17,850 Da |
| NCBI Full Name: | Transcription factor MafG |
| NCBI Synonym Full Names: | MAF bZIP transcription factor G |
| NCBI Official Symbol: | MAFG�� |
| NCBI Official Synonym Symbols: | hMAF�� |
| NCBI Protein Information: | transcription factor MafG |
| UniProt Protein Name: | Transcription factor MafG |
| UniProt Synonym Protein Names: | V-maf musculoaponeurotic fibrosarcoma oncogene homolog G; hMAF |
| Protein Family: | Transcription factor |
| UniProt Gene Name: | MAFG�� |
| UniProt Entry Name: | MAFG_HUMAN |
