Human MFGE8 (Milk Fat Globule EGF Factor 8) ELISA Kit
The Human MFGE8 (Milk Fat Globule EGF Factor 8) ELISA Kit is a highly sensitive and specific assay designed for the accurate detection of MFGE8 levels in human samples including serum, plasma, and cell culture supernatants. This kit provides reliable and reproducible results, making it an ideal tool for a variety of research applications.MFGE8, also known as lactadherin, is a multifunctional protein involved in various biological processes, including phagocytosis, inflammation, and tissue repair. It plays a crucial role in maintaining tissue homeostasis and immune regulation. Dysregulation of MFGE8 has been implicated in conditions such as cancer, inflammatory diseases, and autoimmune disorders, making it a valuable biomarker for studying these pathologies and potential therapeutic interventions.With its high sensitivity and specificity, the Human MFGE8 ELISA Kit offers researchers a powerful tool for quantitative analysis of MFGE8 levels in human samples, further advancing our understanding of its roles in health and disease.
Product Name:
Human MFGE8 (Milk Fat Globule EGF Factor 8) ELISA Kit
SKU:
HUES02979
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Sandwich
Assay type:
Sandwich-ELISA
Assay time:
3 h 30 min
Sensitivity:
37.5 pg/mL
Detection range:
62.5-4000 pg/mL
Reovery:
80%-120%
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to the target protein. Standards or samples are added to the micro ELISA plate wells and bind to the immobilized antibody. A biotinylated detection antibody specific to the target protein is then added, followed by Avidin-Horseradish Peroxidase (HRP) conjugate. Free components are washed away. The substrate solution is added to each well, resulting in a color change. Only wells containing the target protein, detection antibody, and HRP conjugate will develop a blue color. The reaction is terminated by the addition of stop solution, resulting in a yellow color. The optical density (OD) is measured at 450 nm ± 2 nm. The OD value is directly proportional to the concentration of the target protein in the sample and is determined using a standard curve.
