Human MMP-1 (Matrix Metalloproteinase 1) ELISA Kit
The Human MMP-1 (Matrix Metalloproteinase 1) ELISA Kit is expertly crafted for the quantitative detection and measurement of Matrix Metalloproteinase-1 levels in various biological samples. MMP-1, an essential member of the matrix metalloproteinase family, plays a pivotal role in extracellular matrix remodeling and degradation, associated with tissue repair, cellular migration, and various physiological and pathological processes. Accurate quantification of MMP-1 with this ELISA kit provides valuable insights into tissue remodeling, wound healing, and pathological conditions such as cancer metastasis, arthritis, and cardiovascular diseases.
The kit ensures exceptional sensitivity and specificity, delivering reliable and reproducible results. With stringent quality control measures, this kit offers robust performance and ease of use, making it an ideal choice for both research and clinical applications. Trust the Human MMP-1 ELISA Kit for precise and dependable measurement of this critical biomarker in your scientific investigations.
Product Name:
Human MMP-1 (Matrix Metalloproteinase 1) ELISA Kit
SKU:
AEES00109
Target:
Human MMP-1 (Matrix Metalloproteinase 1)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human MMP-1. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human MMP-1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human MMP-1, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human MMP-1. You can calculate the concentration of Human MMP-1 in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
90-103
85-93
89-100
Average (%)
95
90
92
1:4
Range (%)
90-102
94-105
87-99
Average (%)
96
102
92
1:8
Range (%)
90-98
84-99
92-105
Average (%)
94
92
97
1:16
Range (%)
94-102
89-99
95-111
Average (%)
98
96
103
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
82-98
90
EDTA plasma (n=5)
89-102
95
Cell culture media (n=5)
98-108
105
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
85.33
246.42
880.11
82.77
242.81
921.97
Standard deviation
4.95
11.58
36.08
4.59
11.07
47.02
C V (%)
5.8
4.7
4.1
5.55
4.56
5.1
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human MMP-1 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human MMP-1 in samples. No significant cross-reactivity or interference between Human MMP-1 and analogues was observed.
