Human MMP-12 (Matrix Metalloproteinase 12) ELISA Kit
The Human MMP-12 (Matrix Metalloproteinase-12) ELISA Kit is a specialized assay designed for the precise measurement of MMP-12 levels in human biological samples such as serum, plasma, and cell culture supernatants. This kit is known for its high sensitivity and specificity, ensuring accurate and reproducible results that are essential for various research applications.MMP-12, also known as macrophage elastase, is a key enzyme involved in tissue remodeling and inflammation. It plays a crucial role in processes such as extracellular matrix degradation, cell migration, and immune response modulation. Dysregulation of MMP-12 has been implicated in various diseases including chronic obstructive pulmonary disease (COPD), asthma, and atherosclerosis, highlighting its significance as a potential biomarker for studying and developing therapies for these conditions.Overall, the Human MMP-12 ELISA Kit offers researchers a valuable tool for studying the role of MMP-12 in disease pathology and potential therapeutic interventions. Its reliability and accuracy make it an essential component for any laboratory conducting research in the field of matrix metalloproteinases.
Product Name:
Human MMP-12 (Matrix Metalloproteinase 12) ELISA Kit
SKU:
HUES01410
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Sandwich
Assay type:
Sandwich-ELISA
Assay time:
3 h 30 min
Sensitivity:
0.19 ng/mL
Detection range:
0.31-20 ng/mL
Reovery:
80%-120%
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to the target protein. Standards or samples are added to the micro ELISA plate wells and bind to the immobilized antibody. A biotinylated detection antibody specific to the target protein is then added, followed by Avidin-Horseradish Peroxidase (HRP) conjugate. Free components are washed away. The substrate solution is added to each well, resulting in a color change. Only wells containing the target protein, detection antibody, and HRP conjugate will develop a blue color. The reaction is terminated by the addition of stop solution, resulting in a yellow color. The optical density (OD) is measured at 450 nm ± 2 nm. The OD value is directly proportional to the concentration of the target protein in the sample and is determined using a standard curve.
