Human MMP-13 (Matrix Metalloproteinase 13) ELISA Kit
The Matrix Metalloproteinase 13 (MMP-13) ELISA Kit is designed to accurately quantify MMP-13 levels in human samples. MMP-13, a collagenase enzyme that plays a crucial role in extracellular matrix remodeling and tissue maintenance, is associated with various biological processes, including tissue repair, inflammation, and cancer metastasis. Elevated levels of MMP-13 have been linked to pathological conditions such as arthritis, cancer invasion, and wound healing abnormalities. With exceptional sensitivity and specificity, the MMP-13 ELISA Kit provides reliable and reproducible results, enabling precise measurement of MMP-13 concentrations in biological samples.
Manufactured under strict quality controls, this kit ensures robust performance and ease of use, making it ideal for both research and clinical applications. Trust Assay Genie's MMP-13 ELISA Kit for accurate and consistent quantification of this important biomarker in your studies.
Product Name:
Human MMP-13 (Matrix Metalloproteinase 13) ELISA Kit
SKU:
AEES00059
Target:
Human MMP-13 (Matrix Metalloproteinase 13)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
9.38 pg/mL
Detection range:
15.63-1000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human MMP-13. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human MMP-13 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human MMP-13, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human MMP-13. You can calculate the concentration of Human MMP-13 in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
95-109
86-96
90-101
Average (%)
101
91
97
1:4
Range (%)
84-95
92-103
98-106
Average (%)
91
96
102
1:8
Range (%)
97-104
92-105
89-102
Average (%)
101
100
96
1:16
Range (%)
87-101
96-110
93-99
Average (%)
94
104
96
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
95-106
98
EDTA plasma (n=5)
98-110
105
Cell culture media (n=5)
90-103
96
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
43.41
107.9
450.13
44.04
101.12
413.83
Standard deviation
2.34
4.95
22.82
2.53
5.06
19.66
C V (%)
5.39
4.59
5.07
5.74
5.0
4.75
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human MMP-13 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human MMP-13 in samples. No significant cross-reactivity or interference between Human MMP-13 and analogues was observed.
Asparuh Nikolov et al.
The impact of family history of preeclampsia and alteration of MMP-13/TIMP-1 balance in the occurrence of preeclampsia
Journal of Experimental and Clinical Medicine 2022
