Human MMP-14 (Matrix Metalloproteinase 14) ELISA Kit
The Human MMP-14 (Matrix Metalloproteinase-14) ELISA Kit is specifically designed for the precise measurement of MMP-14 levels in human serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, ensuring accurate and consistent results for a variety of research purposes.MMP-14 is a key enzyme involved in extracellular matrix remodeling, and its dysregulation is linked to various pathological conditions including cancer, tissue damage, and inflammatory diseases. By detecting MMP-14 levels, researchers can gain valuable insights into disease mechanisms and potentially identify new therapeutic targets.Overall, the Human MMP-14 ELISA Kit from AssayGenie is a valuable tool for studying MMP-14 biology and its implications in disease, offering researchers a reliable and efficient method for quantifying MMP-14 levels in human samples.
Product Name:
Human MMP-14 (Matrix Metalloproteinase 14) ELISA Kit
SKU:
HUES03318
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Sandwich
Assay type:
Sandwich-ELISA
Assay time:
3 h 30 min
Sensitivity:
0.38 ng/mL
Detection range:
0.63-40 ng/mL
Reovery:
80%-120%
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to the target protein. Standards or samples are added to the micro ELISA plate wells and bind to the immobilized antibody. A biotinylated detection antibody specific to the target protein is then added, followed by Avidin-Horseradish Peroxidase (HRP) conjugate. Free components are washed away. The substrate solution is added to each well, resulting in a color change. Only wells containing the target protein, detection antibody, and HRP conjugate will develop a blue color. The reaction is terminated by the addition of stop solution, resulting in a yellow color. The optical density (OD) is measured at 450 nm ± 2 nm. The OD value is directly proportional to the concentration of the target protein in the sample and is determined using a standard curve.
