Human MMP-9 (Matrix Metalloproteinase 9) ELISA Kit
The Human MMP-9 (Matrix Metalloproteinase 9) ELISA Kit is expertly designed for the precise and quantitative assessment of Matrix Metalloproteinase 9 levels in a variety of biological samples. Matrix Metalloproteinase 9, a crucial enzyme, is known for its role in extracellular matrix remodeling, tissue repair, and a spectrum of physiological and pathological processes. This ELISA kit enables accurate detection of MMP-9, providing valuable insights into processes such as tissue remodeling, cell migration, and inflammation.
With exceptional sensitivity and specificity, the kit ensures reliable and reproducible results. Manufactured under strict quality control measures, this kit offers robust performance and user-friendly protocols, making it an excellent choice for both research and clinical applications. Trust the Human MMP-9 ELISA Kit for accurate and dependable quantification of this vital enzyme in your studies.
Product Name:
Human MMP-9 (Matrix Metalloproteinase 9) ELISA Kit
SKU:
AEES00111
Target:
Human MMP-9 (Matrix Metalloproteinase 9)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
0.10 ng/mL
Detection range:
0.16-10 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human MMP-9. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human MMP-9 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human MMP-9, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human MMP-9. You can calculate the concentration of Human MMP-9 in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
86-99
87-96
100-108
Average (%)
91
90
104
1:4
Range (%)
82-94
83-97
86-101
Average (%)
90
91
94
1:8
Range (%)
97-110
94-107
98-106
Average (%)
103
99
101
1:16
Range (%)
99-111
97-113
90-99
Average (%)
103
105
95
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
87-97
93
EDTA plasma (n=5)
98-110
103
Cell culture media (n=5)
90-102
95
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
0.47
1.05
4.76
0.48
1.15
4.56
Standard deviation
0.03
0.05
0.2
0.03
0.06
0.19
C V (%)
6.38
4.76
4.2
6.25
5.22
4.17
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human MMP-9 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human MMP-9 in samples. No significant cross-reactivity or interference between Human MMP-9 and analogues was observed.
