The Human MT (Melatonin) ELISA Kit is specifically designed for the quantitative detection of melatonin levels in human serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, ensuring accurate and reproducible results for various research applications.Melatonin is a hormone produced by the pineal gland that regulates the sleep-wake cycle and has antioxidant properties. It plays a critical role in maintaining circadian rhythm and promoting healthy sleep patterns. Abnormal melatonin levels have been linked to sleep disorders, mood disorders, and other health conditions, making it a valuable biomarker for studying these conditions and developing potential treatments. This ELISA kit provides a reliable tool for researchers to investigate the physiological functions and clinical implications of melatonin in health and disease. Its easy-to-use format and high-performance characteristics make it an essential asset for any laboratory conducting melatonin-related research.
Product Name:
Human MT (Melatonin) ELISA Kit
SKU:
HUES02946
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Competitive
Assay type:
Competitive-ELISA
Assay time:
2 h 30 min
Sensitivity:
9.38 pg/mL
Detection range:
15.63-1000 pg/mL
Reovery:
80%-120%
This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with the target antigen. Standards or samples are added along with a biotinylated detection antibody. The target antigen present in the sample competes with the immobilized antigen for binding to the detection antibody. After incubation, Avidin-Horseradish Peroxidase (HRP) conjugate is added. Free components are washed away. The substrate solution is then added, resulting in a color change. The intensity of the color is inversely proportional to the concentration of the target antigen in the sample. The reaction is stopped by the addition of stop solution, and the color changes from blue to yellow. The optical density (OD) is measured at 450 nm ± 2 nm. The concentration of the target protein is calculated by comparing the OD values of the samples to the standard curve.
