The Human MTL (Motilin) ELISA Kit is specially designed to detect motilin levels in human biological samples such as serum, plasma, and cell culture supernatants. With a focus on high sensitivity and specificity, this kit provides accurate and reliable results for various research applications.Motilin, a peptide hormone, plays a vital role in gastrointestinal motility by stimulating the contraction of smooth muscles in the digestive system. Dysregulation of motilin levels has been linked to disorders such as gastric stasis and other gastrointestinal issues, making it a valuable biomarker for studying and potentially treating these conditions.
By utilizing the Human MTL ELISA Kit, researchers can gain valuable insights into the role of motilin in gastrointestinal health and diseases, paving the way for advancements in diagnosis and treatment options. Trust in this kit for precise measurements and impactful discoveries in the field of gastrointestinal research.
Product Name:
Human MTL (Motilin) ELISA Kit
SKU:
HUES02973
Target:
Human MTL (Motilin)
Size:
96T
Assay type:
Competitive-ELISA
Assay time:
2.0h
Sensitivity:
9.38 pg/mL
Detection range:
15.63-1000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with MTL. During the reaction, MTL in the sample or standard competes with a fixed amount of MTL on the solid phase supporter for sites on the Biotinylated Detection Ab specific to MTL. Excess conjugate and unbound sample or standard are washed away, and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns from blue to yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of MTL in tested samples can be calculated by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
90-101
89-102
94-108
Average (%)
95
94
100
1:4
Range (%)
90-103
93-104
100-117
Average (%)
96
99
107
1:8
Range (%)
89-104
88-100
97-112
Average (%)
95
95
103
1:16
Range (%)
85-97
93-107
99-112
Average (%)
91
99
106
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
87-101
94
EDTA plasma (n=5)
88-100
93
Cell culture media (n=5)
92-106
98
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
51.3
135.8
450.5
48.5
128.6
437.5
Standard deviation
3.1
8.0
15.3
3.2
5.4
22.8
C V (%)
6.04
5.89
3.4
6.6
4.2
5.21
Sample type &Sample volume:
serum, plasma and other biological fluids; 50μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of MTL concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes MTL in samples. No significant cross-reactivity or interference between MTL and analogues was observed.
