Human mTOR (Mammalian Target of Rapamycin) CLIA Kit
The Human mTOR (Mammalian Target of Rapamycin) CLIA Kit is specifically designed for the accurate detection of mTOR levels in human samples such as serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, ensuring reliable and reproducible results for various research applications.mTOR is a vital protein that regulates cell growth, proliferation, and metabolism, playing a crucial role in various cellular processes.
Dysregulation of mTOR has been linked to numerous diseases, including cancer, diabetes, and neurodegenerative disorders, making it a valuable biomarker for studying these conditions and developing potential treatments.Overall, the Human mTOR CLIA Kit from AssayGenie provides researchers with a powerful tool to investigate the role of mTOR in health and disease, facilitating advancements in scientific research and potential therapeutic strategies.
Product Name:
Human mTOR (Mammalian Target of Rapamycin) CLIA Kit
SKU:
HUES00931
Target:
Human mTOR (Mammalian Target of Rapamycin)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human mTOR. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human mTOR and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human mTOR, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human mTOR. You can calculate the concentration of Human mTOR in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
86-102
84-99
93-105
Average (%)
93
91
99
1:4
Range (%)
87-103
100-117
86-100
Average (%)
94
108
93
1:8
Range (%)
100-114
99-113
98-115
Average (%)
108
104
106
1:16
Range (%)
88-103
99-116
88-100
Average (%)
96
107
94
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
100-112
106
EDTA plasma (n=5)
94-105
100
Cell culture media (n=5)
92-104
99
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
96.68
278.01
899.95
87.25
253.37
883.59
Standard deviation
8.18
21.21
95.39
9.82
27.24
89.86
C V (%)
8.46
7.63
10.6
11.26
10.75
10.17
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human mTOR concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human mTOR in samples. No significant cross-reactivity or interference between Human mTOR and analogues was observed.
