The Human Myo/Myoglobin CLIA Kit is specifically designed for the precise quantification of myoglobin levels in human biological samples such as serum and plasma. With its high sensitivity and specificity, this kit provides accurate and dependable results, making it suitable for various research applications.Myoglobin is a key protein that is found in skeletal and cardiac muscle tissues, serving as an important marker for muscle injury and related disorders.
Elevated levels of myoglobin are commonly associated with conditions such as myocardial infarction and rhabdomyolysis, making it a valuable biomarker for monitoring and diagnosing these conditions.Overall, the Human Myo/Myoglobin CLIA Kit offers researchers a reliable tool for studying muscle-related disorders and exploring potential therapeutic interventions.
Product Name:
Human MYO (Myoglobin) CLIA Kit
SKU:
HUES00126
Target:
Human MYO (Myoglobin)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
0.38 ng/mL
Detection range:
0.63-40 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human MYO. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human MYO and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human MYO, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human MYO. You can calculate the concentration of Human MYO in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
87-99
85-97
97-111
Average (%)
93
91
105
1:4
Range (%)
86-98
97-114
97-112
Average (%)
91
105
105
1:8
Range (%)
93-105
101-113
93-107
Average (%)
98
107
100
1:16
Range (%)
97-112
93-108
84-98
Average (%)
104
100
91
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
89-102
95
EDTA plasma (n=5)
88-103
96
Cell culture media (n=5)
100-113
107
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
2.22
5.24
18.71
2.2
5.39
20.37
Standard deviation
0.26
0.61
1.39
0.27
0.54
2.23
C V (%)
11.71
11.64
7.43
12.27
10.02
10.95
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human MYO concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human MYO in samples. No significant cross-reactivity or interference between Human MYO and analogues was observed.
