Human NIT2 Recombinant Protein (RPPB2023)
- SKU:
- RPPB2023
- Product Type:
- Recombinant Protein
- Species:
- Human
- Uniprot:
- Q9NQR4
- Research Area:
- Enzymes
Description
| Product Name: | Human NIT2 Recombinant Protein |
| Product Code: | RPPB2023 |
| Size: | 10µg |
| Species: | Human |
| Target: | NIT2 |
| Synonyms: | Nitrilase homolog 2, Nitrilase family member 2, omega-amidase NIT2, MGC111199, Nit protein 2, EC 3.5.1.3. |
| Source: | Escherichia Coli |
| Physical Appearance: | Sterile Filtered clear solution. |
| Formulation: | The NIT2 protein solution (1mg/1ml) is formulated in 20mM Tris-HCl buffer (pH 8.0) 1mM DTT, 0.1M NaCl, and 10% glycerol. |
| Stability: | Store at 4°C if entire vial will be used within 2-4 weeks.�Store, frozen at -20°C for longer periods of time.For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please avoid freeze thaw cycles. |
| Purity: | Greater than 95% as determined by SDS-PAGE. |
| Amino Acid Sequence: | MGSSHHHHHH SSGLVPRGSH MGSMTSFRLA LIQLQISSIK SDNVTRACSF IREAATQGAK IVSLPECFNS PYGAKYFPEY AEKIPGESTQ KLSEVAKECS IYLIGGSIPE EDAGKLYNTC AVFGPDGTLL AKYRKIHLFD IDVPGKITFQ ESKTLSPGDS FSTFDTPYCR VGLGICYDMR FAELAQIYAQ RGCQLLVYPG AFNLTTGPAH WELLQRSRAV DNQVYVATAS PARDDKASYV AWGHSTVVNP WGEVLAKAGT EEAIVYSDID LKKLAEIRQQ IPVFRQKRSD LYAVEMKKP |
NIT2 is a member of the nitrilase superfamily. NIT2 protein has an omega-amidase activity which removes potentially toxic intermediates by converting alpha-ketoglutaramate and alpha-ketosuccinamate to biologically useful alpha-ketoglutarate and oxaloacetate, respectively. In addition, Nit2 is widely distributed in nature and is thought to be a tumor suppressor protein.
NIT2 produced in E.Coli is a single, non-glycosylated polypeptide chain containing 299 amino acids (1-276a.a.) and having a molecular mass of 33kDa.NIT2 is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
| UniProt Protein Function: | NIT2: a protein of unknown function. A CN hydrolase domain suggests that this protein may be involved in nitrilase-related metabolism and handling. |
| UniProt Protein Details: | Protein type:EC 3.5.1.3; Hydrolase; Mitochondrial Chromosomal Location of Human Ortholog: 3q12.2 Cellular Component: centrosome; mitochondrion; cytoplasm Molecular Function:omega-amidase activity Biological Process: oxaloacetate metabolic process; glutamine metabolic process; asparagine metabolic process |
| UniProt Code: | Q9NQR4 |
| NCBI GenInfo Identifier: | 74725271 |
| NCBI Gene ID: | 56954 |
| NCBI Accession: | Q9NQR4.1 |
| UniProt Secondary Accession: | Q9NQR4,Q8WUF0, B2R9A3, D3DN47, |
| UniProt Related Accession: | Q9NQR4 |
| Molecular Weight: | 276 |
| NCBI Full Name: | Omega-amidase NIT2 |
| NCBI Synonym Full Names: | nitrilase family, member 2 |
| NCBI Official Symbol: | NIT2�� |
| NCBI Official Synonym Symbols: | HEL-S-8a�� |
| NCBI Protein Information: | omega-amidase NIT2; Nit protein 2; nitrilase homolog 2; epididymis secretory sperm binding protein Li 8a |
| UniProt Protein Name: | Omega-amidase NIT2 |
| UniProt Synonym Protein Names: | Nitrilase homolog 2 |
| Protein Family: | Nitrilase |
| UniProt Gene Name: | NIT2�� |
| UniProt Entry Name: | NIT2_HUMAN |
