The Human NSE (Neuron-Specific Enolase) ELISA Kit is specifically designed for the quantification of NSE levels in human serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, guaranteeing accurate and consistent results for various research purposes. Neuron-Specific Enolase is a key enzyme found in neurons and neuroendocrine cells, serving as a valuable marker for neuronal damage and neurodegenerative disorders. Elevated levels of NSE have been associated with conditions like stroke, traumatic brain injury, and neuroblastoma, highlighting its importance in disease diagnosis and monitoring. With its reliable performance and ease of use, the Human NSE ELISA Kit is an indispensable tool for scientists and researchers investigating neurologic diseases and seeking novel therapeutic approaches. Get your kit today and unlock the potential of NSE in advancing your research endeavors.
Product Name:
Human NSE (Neuron-Specific Enolase) ELISA Kit
SKU:
HUES02147
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Sandwich
Assay type:
Sandwich-ELISA
Assay time:
3 h 30 min
Sensitivity:
1.4 ng/mL
Detection range:
2.34-150 ng/mL
Reovery:
80%-120%
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to the target protein. Standards or samples are added to the micro ELISA plate wells and bind to the immobilized antibody. A biotinylated detection antibody specific to the target protein is then added, followed by Avidin-Horseradish Peroxidase (HRP) conjugate. Free components are washed away. The substrate solution is added to each well, resulting in a color change. Only wells containing the target protein, detection antibody, and HRP conjugate will develop a blue color. The reaction is terminated by the addition of stop solution, resulting in a yellow color. The optical density (OD) is measured at 450 nm ± 2 nm. The OD value is directly proportional to the concentration of the target protein in the sample and is determined using a standard curve.
